Literature summary extracted from
Singh, M.; Böttger, B.; Stewart, C.; Brooks, G.C.; Srere, P.A.
S-Acetyl phosphopantetheine: deacetyl citrate lyase S-acetyl transferase from Klebsiella aerogenes (1973), Biochem. Biophys. Res. Commun., 53, 1-9.
General Stability
EC Number |
General Stability |
Organism |
---|
2.3.1.49 |
DTT stabilizes |
Klebsiella aerogenes |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.3.1.49 |
N-ethylmaleimide |
preincubation of the acetyl transferase with 0.4 mM N-ethylmaleimide results in large decrease of active citrate lyase formed |
Klebsiella aerogenes |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.49 |
Klebsiella aerogenes |
- |
- |
- |
Oxidation Stability
EC Number |
Oxidation Stability |
Organism |
---|
2.3.1.49 |
O2-sensitive, DTT stabilizes |
Klebsiella aerogenes |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.3.1.49 |
partial |
Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.3.1.49 |
17.4 |
- |
- |
Klebsiella aerogenes |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.1.49 |
S-acetylphosphopantetheine + deacetyl-[citrate-oxaloacetate-lyase((pro-3S)-CH2COO---acetate)] |
- |
Klebsiella aerogenes |
phosphopantetheine + [citrate-oxaloacetate-lyase((pro-3S)-CH2COO---acetate)] |
i.e. active form of EC 4.1.3.6 |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.3.1.49 |
25 |
- |
assay at |
Klebsiella aerogenes |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.3.1.49 |
7.7 |
- |
- |
Klebsiella aerogenes |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
2.3.1.49 |
6 |
8 |
25% of maximal activity at pH 6, 95% of maximal activity at pH 8 |
Klebsiella aerogenes |