Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Trievel, R.C.; Li, F.Y.; Marmorstein, R.
    Application of a fluorescent histone acetyltransferase assay to probe the substrate specificity of the human p300/CBP-associated factor (2000), Anal. Biochem., 287, 319-328.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.48 overexpression of enzyme domain PCAF amino acid residues 493-658 in Escherichia coli Homo sapiens
2.3.1.48 overexpression of enzyme domain, tGcn5 consists of amino acid residues 48-210 Tetrahymena thermophila
2.3.1.48 overexpression of enzyme domain, yGcn5 consists of amino acid residues 99-262 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.48 0.75
-
 histone H3-peptide p27 PCAF catalytic domain Homo sapiens
2.3.1.48 1.12
-
 histone H3-peptide p19 PCAF catalytic domain Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.48 additional information
-
-
 Tetrahymena thermophila
2.3.1.48 additional information
-
 three-dimensional structure of the ternary complex of tGcn5 domain Homo sapiens
2.3.1.48 additional information
-
 three-dimensional structure of the ternary complex of tGcn5 domain Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.48 Homo sapiens
-
 GCN5
-
2.3.1.48 Homo sapiens
-
 PCAF protein
-
2.3.1.48 Homo sapiens GCN5
-
 GCN5
-
2.3.1.48 Saccharomyces cerevisiae
-
 gene Gcn5
-
2.3.1.48 Tetrahymena thermophila
-
 GCN5
-
2.3.1.48 Tetrahymena thermophila GCN5
-
 GCN5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.48 recombinant catalytic domain of Gcn5 protein Saccharomyces cerevisiae
2.3.1.48 recombinant enzyme domains of PCAF and Gcn5 Homo sapiens
2.3.1.48 tGcn5 domain, recombinant from E. coli Tetrahymena thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.48 acetyl-CoA + histone
-
 Homo sapiens CoA + acetylhistone
-
 ir
2.3.1.48 acetyl-CoA + histone
-
 Saccharomyces cerevisiae CoA + acetylhistone
-
 ?
2.3.1.48 acetyl-CoA + histone
-
 Tetrahymena thermophila CoA + acetylhistone
-
 ?
2.3.1.48 acetyl-CoA + histone
-
 Homo sapiens GCN5 CoA + acetylhistone
-
 ir
2.3.1.48 acetyl-CoA + histone
-
 Tetrahymena thermophila GCN5 CoA + acetylhistone
-
 ?
2.3.1.48 acetyl-CoA + histone H3
-
 Saccharomyces cerevisiae CoA + acetylhistone H3
-
 ?
2.3.1.48 acetyl-CoA + histone H3 PCAF protein Homo sapiens CoA + acetylhistone H3
-
 ?
2.3.1.48 acetyl-CoA + histone H3 PCAF protein Homo sapiens GCN5 CoA + acetylhistone H3
-
 ?
2.3.1.48 acetyl-CoA + histone H3 peptide peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF Homo sapiens CoA + acetylhistone H3 peptide
-
 ?
2.3.1.48 acetyl-CoA + histone H3 peptide peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF Saccharomyces cerevisiae CoA + acetylhistone H3 peptide
-
 ?
2.3.1.48 acetyl-CoA + histone H3 peptide peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF Tetrahymena thermophila CoA + acetylhistone H3 peptide
-
 ?
2.3.1.48 acetyl-CoA + histone H3 peptide peptide H3p20 Homo sapiens CoA + acetylhistone H3 peptide
-
 ?
2.3.1.48 acetyl-CoA + histone H3 peptide peptide H3p20 Saccharomyces cerevisiae CoA + acetylhistone H3 peptide
-
 ?
2.3.1.48 acetyl-CoA + histone H3 peptide peptide H3p20 Tetrahymena thermophila CoA + acetylhistone H3 peptide
-
 ?
2.3.1.48 acetyl-CoA + histone H3 peptide peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF Homo sapiens GCN5 CoA + acetylhistone H3 peptide
-
 ?
2.3.1.48 acetyl-CoA + histone H3 peptide peptide H3p20 Homo sapiens GCN5 CoA + acetylhistone H3 peptide
-
 ?
2.3.1.48 acetyl-CoA + histone H3 peptide peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF Tetrahymena thermophila GCN5 CoA + acetylhistone H3 peptide
-
 ?
2.3.1.48 acetyl-CoA + histone H3 peptide peptide H3p20 Tetrahymena thermophila GCN5 CoA + acetylhistone H3 peptide
-
 ?
2.3.1.48 acetyl-CoA + histone H3-peptide p19
-
 Homo sapiens CoA + acetylhistone H3-peptide p19
-
 ?
2.3.1.48 acetyl-CoA + histone H3-peptide p19
-
 Homo sapiens CoA + acetylhistone H3 -peptide p19
-
 ?
2.3.1.48 acetyl-CoA + histone H3-peptide p27
-
 Homo sapiens CoA + acetylhistone H3 -peptide p27
-
 ?
2.3.1.48 acetyl-CoA + histone H4
-
 Saccharomyces cerevisiae CoA + acetylhistone H4
-
 ?
2.3.1.48 acetyl-CoA + histone H4
-
 Tetrahymena thermophila CoA + acetylhistone H4
-
 ?
2.3.1.48 acetyl-CoA + histone H4 PCAF protein Homo sapiens CoA + acetylhistone H4
-
 ?
2.3.1.48 acetyl-CoA + histone H4 PCAF protein Homo sapiens GCN5 CoA + acetylhistone H4
-
 ?
2.3.1.48 acetyl-CoA + histone H4
-
 Tetrahymena thermophila GCN5 CoA + acetylhistone H4
-
 ?
2.3.1.48 acetyl-CoA + protein p53 peptide of p53 is a substrate for PCAF catalytic domain Homo sapiens CoA + acetylprotein p53
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.48 HAT
-
 Homo sapiens
2.3.1.48 HAT
-
 Saccharomyces cerevisiae
2.3.1.48 HAT
-
 Tetrahymena thermophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.48 0.131
-
 histone H3-peptide p19 PCAF catalytic domain Homo sapiens
2.3.1.48 0.17
-
 histone H3-peptide p27 PCAF catalytic domain Homo sapiens