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Literature summary extracted from

  • Ohba, R.; Steger, D.J.; Brownell, J.E.; Mizzen, C.A.; Cook, R.G.; Cote, J.; Workman, J.L.; Allis, C.D.
    A novel H2A/H4 nucleosomal histone acetyltransferase in Tetrahymena thermophila (1999), Mol. Cell. Biol., 19, 2061-2068.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.48 nucleus macronucleus, enzyme form A Tetrahymena thermophila 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.48 80000
-
 NuA4-like enzyme, sucrose density gradient centrifugation Tetrahymena thermophila

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.48 Saccharomyces cerevisiae
-
 nucleosomal H2A/H4 acetyltransferase, i.e. NuA4 protein
-
2.3.1.48 Tetrahymena thermophila
-
 yeast-NuA4-like enzyme form
-
2.3.1.48 Tetrahymena thermophila
-
 transcription-associated enzyme form A, containing protein p55 as catalytic subunit
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.48 acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine acetylation of lysine 5, 8, 12, and 16 of free histone H4 Saccharomyces cerevisiae
a
2.3.1.48 acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine acetylation of lysine 5, 8, 12, and 16 of free histone H4 Tetrahymena thermophila
a

Storage Stability

EC Number Storage Stability Organism
2.3.1.48 -70°C, crude extract, 25mM Tris-HCl, after treatment with DNase I, 10% glycerol, stable for months Tetrahymena thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.48 acetyl-CoA + histone
-
 Saccharomyces cerevisiae CoA + acetylhistone
-
 ?
2.3.1.48 acetyl-CoA + histone
-
 Tetrahymena thermophila CoA + acetylhistone
-
 ?
2.3.1.48 acetyl-CoA + histone H2A NuA4-like protein acetylates histone H4 and H2A Saccharomyces cerevisiae CoA + acetylhistone H2A
-
 ?
2.3.1.48 acetyl-CoA + histone H2A NuA4-like protein acetylates histone H4 and H2A Tetrahymena thermophila CoA + acetylhistone H2A
-
 ?
2.3.1.48 acetyl-CoA + histone H4 NuA4-like protein acetylates histone H4 and H2A Saccharomyces cerevisiae CoA + acetylhistone H4
-
 ?
2.3.1.48 acetyl-CoA + histone H4 NuA4-like protein acetylates histone H4 and H2A Tetrahymena thermophila CoA + acetylhistone H4
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.48 HAT
-
 Saccharomyces cerevisiae
2.3.1.48 HAT
-
 Tetrahymena thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.48 30
-
 NuA4-like protein, assay at Tetrahymena thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.48 8
-
 assay at Tetrahymena thermophila