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Literature summary extracted from

  • Travis, G.H.; Colavito-Shepanski, M.; Grunstein, M.
    Extensive purification and characterization of chromatin-bound histone acetyltransferase from Saccharomyces cerevisiae (1984), J. Biol. Chem., 259, 14406-14412.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.48 NaCl, 1.0 M, stable for several days Saccharomyces cerevisiae
2.3.1.48 sensitive to freezing Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.48 Ca2+ weak Saccharomyces cerevisiae
2.3.1.48 CoA reversible Saccharomyces cerevisiae
2.3.1.48 Mg2+ weak Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.48 0.0009
-
 acetyl-CoA
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.48 chromatin
-
 Saccharomyces cerevisiae 785
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.48 NaCl
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.48 110000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.48 Saccharomyces cerevisiae
-
 enzyme form A and B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.48
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.48 0.068
-
 purified enzyme Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.3.1.48 -20°C, 50% glycerol, stable for several months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.48 acetyl-CoA + histone a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms Saccharomyces cerevisiae CoA + acetylhistone
-
 r
2.3.1.48 acetyl-CoA + histone H4 histone H4 is the preferred substrate Saccharomyces cerevisiae CoA + acetylhistone H4
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.48 25
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.48 60
-
 1 min, irreversible denaturation Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.48 8.2
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.48 6.5 9.5 less than 10% of activity maximum below pH 6.5 and above pH 9.5 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.48 6 10 irreversible loss of activity below pH 6.0 and above pH 10.0 Saccharomyces cerevisiae