EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.180 | pharmacology | the enzyme is a target for design and development of antibacterial drugs against pathogenic bacteria | Streptococcus pneumoniae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.41 | expression as His-tagged protein in Escherichia coli | Haemophilus influenzae |
2.3.1.41 | overexpression of His-tagged FabH in Escherichia coli BL21 (DE3) | Streptococcus pneumoniae |
2.3.1.180 | gene fabH, expression of His-tagged enzyme containing a thrombin cleavage site in strain BL21(DE3) | Streptococcus pneumoniae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.41 | cerulenin | - |
Escherichia coli | |
2.3.1.41 | cerulenin | - |
Haemophilus influenzae | |
2.3.1.41 | cerulenin | - |
Streptococcus pneumoniae | |
2.3.1.41 | SB418011 | - |
Escherichia coli | |
2.3.1.41 | SB418011 | - |
Haemophilus influenzae | |
2.3.1.41 | SB418011 | strong, synthase III | Streptococcus pneumoniae | |
2.3.1.41 | thiolactomycin | synthase III | Escherichia coli | |
2.3.1.41 | thiolactomycin | - |
Haemophilus influenzae | |
2.3.1.41 | thiolactomycin | synthase III; weak | Streptococcus pneumoniae | |
2.3.1.180 | cerulenin | the IC50 is 0.2 mM | Streptococcus pneumoniae | |
2.3.1.180 | SB418011 | potent inhibition, the IC50 is 16 nM | Streptococcus pneumoniae | |
2.3.1.180 | thiolactomycin | weak inhibition, the IC50 is 0.008 mM | Streptococcus pneumoniae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.41 | 0.0186 | - |
malonyl-[acyl-carrier protein] | synthase III | Streptococcus pneumoniae | |
2.3.1.41 | 0.0403 | - |
acetyl-CoA | synthase III | Streptococcus pneumoniae | |
2.3.1.180 | 0.0186 | - |
malonyl-[acyl-carrier protein] | pH 7.0, recombinant enzyme | Streptococcus pneumoniae | |
2.3.1.180 | 0.0403 | - |
acetyl-CoA | pH 7.0, recombinant enzyme | Streptococcus pneumoniae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.41 | additional information | - |
amino acid sequence alignment | Haemophilus influenzae |
2.3.1.41 | additional information | - |
amino acid sequence alignment | Escherichia coli |
2.3.1.41 | additional information | - |
amino acid sequence alignment | Streptococcus pneumoniae |
2.3.1.41 | 60000 | - |
synthase III, gel filtration | Streptococcus pneumoniae |
2.3.1.180 | 34000 | - |
x * 34000, recombinant detagged enzyme, SDS-PAGE | Streptococcus pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | Streptococcus pneumoniae | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.41 | Escherichia coli | - |
- |
- |
2.3.1.41 | Haemophilus influenzae | - |
- |
- |
2.3.1.41 | Streptococcus pneumoniae | P0A3C5 | synthase III, gene fabH | - |
2.3.1.180 | Streptococcus pneumoniae | P0A3C5 | gene fabH | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.41 | recombinant His-tagged protein from E. coli | Haemophilus influenzae |
2.3.1.41 | recombinant His-tagged synthase III from E. coli | Streptococcus pneumoniae |
2.3.1.180 | recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography and gel filtration, the tag is cleaved off by thrombin | Streptococcus pneumoniae |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | synthaseIII/FabH: His-Asn-Cys catalytic triad | Haemophilus influenzae | |
2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | synthaseIII/FabH: His-Asn-Cys catalytic triad | Escherichia coli | |
2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | synthaseIII/FabH: His-Asn-Cys catalytic triad | Streptococcus pneumoniae | |
2.3.1.180 | acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | active site structure contains a catalytic triad of His249-Asn279-Cys112 residues | Streptococcus pneumoniae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.41 | 0.005 | - |
synthase III | Streptococcus pneumoniae |
2.3.1.180 | additional information | - |
substrate specificity | Streptococcus pneumoniae |
2.3.1.180 | 0.005 | - |
purified recombinant enzyme, substrates acetyl-CoA and malonyl-[acyl-carrier-protein] | Streptococcus pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.41 | acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Haemophilus influenzae | acetoacyl-[acyl-carrier protein] + CO2 + CoA | - |
? | |
2.3.1.41 | acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Escherichia coli | acetoacyl-[acyl-carrier protein] + CO2 + CoA | - |
? | |
2.3.1.41 | acetyl-CoA + malonyl-[acyl-carrier protein] | synthase III, best substrate | Streptococcus pneumoniae | acetoacyl-[acyl-carrier protein] + CO2 + CoA | - |
? | |
2.3.1.41 | additional information | - |
Escherichia coli | ? | - |
? | |
2.3.1.41 | additional information | synthase III, FabH, has a preference for straight short-chain CoA primers | Streptococcus pneumoniae | ? | - |
? | |
2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Haemophilus influenzae | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Streptococcus pneumoniae | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Streptococcus pneumoniae | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | preferred CoA primer is acetyl-CoA | Streptococcus pneumoniae | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | butyryl-CoA + malonyl-[acyl-carrier protein] | - |
Streptococcus pneumoniae | 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | isobutyryl-CoA + malonyl-[acyl-carrier protein] | - |
Streptococcus pneumoniae | 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | isovaleryl-CoA + malonyl-[acyl-carrier protein] | - |
Streptococcus pneumoniae | 3-oxo-5-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | additional information | substrate specificity, the recombinant enzyme prefers straight short-chain CoA primer substrates, preferred CoA primer is acetyl-CoA, no activity with lauroyl-CoA and palmitoyl-CoA | Streptococcus pneumoniae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.180 | ? | x * 34000, recombinant detagged enzyme, SDS-PAGE | Streptococcus pneumoniae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.180 | beta-ketoacyl-acyl carrier protein synthase III | - |
Streptococcus pneumoniae |
2.3.1.180 | FabH | - |
Streptococcus pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.180 | 7 | - |
assay at | Streptococcus pneumoniae |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.3.1.180 | 0.000016 | - |
potent inhibition, the IC50 is 16 nM | Streptococcus pneumoniae | SB418011 | |
2.3.1.180 | 0.008 | - |
weak inhibition, the IC50 is 0.008 mM | Streptococcus pneumoniae | thiolactomycin | |
2.3.1.180 | 0.2 | - |
the IC50 is 0.2 mM | Streptococcus pneumoniae | cerulenin |