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Literature summary extracted from

  • Choi, K.H.; Heath, R.J.; Rock, C.O.
    beta-ketoacyl-acyl carrier protein synthase III (FabH) is a determining factor in branched-chain fatty acid biosynthesis (2000), J. Bacteriol., 182, 365-370.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.41 expression of His-tagged FabH1 and FabH2 in Escherichia coli INValphaF' Bacillus subtilis
2.3.1.180 genes fabH1 and fabH2, expression of His-tagged enzymes in Escherichia coli strain BL21(DE3) Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 additional information
-
amino acid sequence alignment Bacillus subtilis
2.3.1.41 additional information
-
amino acid sequence alignment Escherichia coli
2.3.1.41 39800
-
x * 39800, FabH2, SDS-PAGE Bacillus subtilis
2.3.1.41 42700
-
x * 42700, FabH1, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Escherichia coli
-
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Bacillus subtilis initiation of fatty acid biosynthesis with acetyl-CoA as a primer, overview substrate specificity 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Bacillus subtilis
-
genes fabH1 and fabH2, 2 synthase III isozymes
-
2.3.1.41 Escherichia coli
-
synthase III, gene fabH
-
2.3.1.180 Bacillus subtilis
-
genes fabH1 and fabH2 encoding isozymes FabH1 and FabH2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 recombinant His-tagged proteins FabH1 and FabH2 from E. coli Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.41 additional information
-
-
Bacillus subtilis
2.3.1.41 additional information
-
-
Escherichia coli
2.3.1.180 additional information
-
substrate specificities of FabH1 and FabH2 Bacillus subtilis
2.3.1.180 0.024
-
purified recombinant His-tagged isoyzme FabH1, primer substrate acetyl-CoA Bacillus subtilis
2.3.1.180 0.105
-
purified recombinant His-tagged isoyzme FabH2, primer substrate acetyl-CoA Bacillus subtilis
2.3.1.180 0.155
-
purified recombinant His-tagged isoyzme FabH1, primer substrate 2-methylbutyryl-CoA Bacillus subtilis
2.3.1.180 0.162
-
purified recombinant His-tagged isoyzme FabH2, primer substrate isovaleryl-CoA Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] initiation of fatty acid biosynthesis with acetyl-CoA as a primer, overview substrate specificity Bacillus subtilis 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 additional information
-
Escherichia coli ?
-
?
2.3.1.41 additional information synthase III isozymes also utilize iso- and anteiso-branched-chain acyl-CoA primers as substrates Bacillus subtilis ?
-
?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Bacillus subtilis 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.180 2-methylbutyryl-CoA + malonyl-[acyl-carrier protein] 2-methylbutyryl-CoA is the preferred primer substrate of isozyme FabH1 Bacillus subtilis 3-oxo-4-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
Bacillus subtilis acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.180 butyryl-CoA + malonyl-[acyl-carrier protein]
-
Bacillus subtilis 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.180 heptanoyl-CoA + malonyl-[acyl-carrier protein]
-
Bacillus subtilis 3-oxo-nonanoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.180 hexanoyl-CoA + malonyl-[acyl-carrier protein]
-
Bacillus subtilis 3-oxo-octanoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.180 isobutyryl-CoA + malonyl-[acyl-carrier protein]
-
Bacillus subtilis 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.180 isovaleryl-CoA + malonyl-[acyl-carrier protein] isovaleryl-CoA is the preferred primer substrate of isozyme FabH2 Bacillus subtilis 3-oxo-5-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.180 additional information substrate specificities of FabH1 and FabH2 with different primer substrates, both isozymes prefer branched-chain fatty acids Bacillus subtilis ?
-
?
2.3.1.180 octanoyl-CoA + malonyl-[acyl-carrier protein] low activity with octanoyl-CoA as primer substrate of isozyme FabH1, no activity with FabH2 Bacillus subtilis 3-oxo-decanoyl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.180 propionyl-CoA + malonyl-[acyl-carrier protein]
-
Bacillus subtilis 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2
-
?
2.3.1.180 valeryl-CoA + malonyl-[acyl-carrier protein]
-
Bacillus subtilis 3-oxo-heptanoyl-[acyl-carrier protein] + CoA + CO2
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.41 ? x * 42700, FabH1, SDS-PAGE Bacillus subtilis
2.3.1.41 ? x * 39800, FabH2, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
Bacillus subtilis
2.3.1.180 FabH
-
Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.41 37
-
assay at Bacillus subtilis
2.3.1.41 37
-
assay at Escherichia coli
2.3.1.180 37
-
assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.41 7
-
assay at Bacillus subtilis
2.3.1.41 7
-
assay at Escherichia coli
2.3.1.180 7
-
assay at Bacillus subtilis