Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Han, L.; Lobo, S.; Reynolds, K.A.
    Characterization of beta-ketoacyl-acyl carrier protein synthase III from Streptomyces glaucescens and its role in initiation of fatty acid biosynthesis (1998), J. Bacteriol., 180, 4481-4486.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.180 synthesis the recombinant enzyme is useful for production of fatty acids by directed biosynthesis profiting of the flexible substrate specificity for acyl-CoAs of the enzyme, overview Streptomyces glaucescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.41 overexpression of fabH in Escherichia coli as His-tagged protein, synthase III Streptomyces glaucescens
2.3.1.180 gene fabH, overexpression of the His-tagged in Escherichia coli Streptomyces glaucescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 thiolactomycin synthase III Streptomyces glaucescens
2.3.1.180 thiolactomycin inhibits both KAS III and ACAT activities Streptomyces glaucescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.41 0.00041
-
 isobutyryl-CoA synthase III Streptomyces glaucescens
2.3.1.41 0.00071
-
 butyryl-CoA synthase III Streptomyces glaucescens
2.3.1.41 0.0024
-
 acetyl-CoA synthase III Streptomyces glaucescens
2.3.1.41 0.0037
-
 malonyl-[acyl-carrier protein] synthase III Streptomyces glaucescens
2.3.1.180 0.0004
-
 isobutyryl-CoA pH 7.0, 30°C, recombinant His-tagged enzyme Streptomyces glaucescens
2.3.1.180 0.0007
-
 butyryl-CoA pH 7.0, 30°C, recombinant His-tagged enzyme Streptomyces glaucescens
2.3.1.180 0.0024
-
 acetyl-CoA pH 7.0, 30°C, recombinant His-tagged enzyme Streptomyces glaucescens
2.3.1.180 0.0037
-
 malonyl-[acyl-carrier protein] pH 7.0, 30°C, recombinant His-tagged enzyme Streptomyces glaucescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 37000
-
 2 * 37000, recombinant synthase III, SDS-PAGE Streptomyces glaucescens
2.3.1.41 72000
-
 recombinant enzyme, gel filtration Streptomyces glaucescens
2.3.1.180 37000
-
 2 * 37000, recombinant His-tagged enzyme, SDS-PAGE Streptomyces glaucescens
2.3.1.180 72000
-
 recombinant His-tagged enzyme, gel filtration Streptomyces glaucescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Streptomyces glaucescens initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 butyryl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens
-
 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 isobutyryl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens
-
 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information Streptomyces glaucescens the enzyme is involved in biosynthesis of straight-chain and branched-chain fatty acids with the specificity for acyl-CoA substrate determining the produced fatty acid spectrum ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Streptomyces glaucescens
-
 synthase III, gene fabH
-
2.3.1.180 Streptomyces glaucescens
-
 gene fabH
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 recombinant from E. coli as His-tagged protein Streptomyces glaucescens
2.3.1.180 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Streptomyces glaucescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens ?
-
 ?
2.3.1.41 acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase III Streptomyces glaucescens acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo Streptomyces glaucescens 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase III Streptomyces glaucescens beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 isobutyryl-CoA + malonyl-[acyl-carrier protein] synthase III Streptomyces glaucescens ?
-
 ?
2.3.1.41 additional information malonyl-CoA-CO2 exchange reaction Streptomyces glaucescens ?
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Streptomyces glaucescens 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase III, broad specificity Streptomyces glaucescens 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 butyryl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 isobutyryl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information the enzyme is involved in biosynthesis of straight-chain and branched-chain fatty acids with the specificity for acyl-CoA substrate determining the produced fatty acid spectrum Streptomyces glaucescens ?
-
 ?
2.3.1.180 additional information the enzyme shows also acyl-CoA:[acyl-carrier-protein] transacylase activity, i.e. ACAT activity, in a ratio of 1:0.12 with beta-oxoacyl-[acyl-carrier-protein] synthase III activity Streptomyces glaucescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.41 dimer 2 * 37000, recombinant synthase III, SDS-PAGE Streptomyces glaucescens
2.3.1.180 dimer 2 * 37000, recombinant His-tagged enzyme, SDS-PAGE Streptomyces glaucescens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.41 KAS
-
 Streptomyces glaucescens
2.3.1.41 More enzyme shows also acetyl coenzyme A-acyl-carrier-protein transacylase activity EC 2.3.1.38, 12% compared to beta-keto-[acyl-carrier-protein] synthase activity EC 2.3.1.41 Streptomyces glaucescens
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
 Streptomyces glaucescens
2.3.1.180 beta-ketoacyl-[acyl-carrier-protein] synthase III
-
 Streptomyces glaucescens
2.3.1.180 FabH
-
 Streptomyces glaucescens
2.3.1.180 KAS III
-
 Streptomyces glaucescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.41 30
-
 assay at Streptomyces glaucescens
2.3.1.180 30
-
 assay at Streptomyces glaucescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.41 6.6 7.7 synthase III Streptomyces glaucescens
2.3.1.41 7
-
 assay at Streptomyces glaucescens
2.3.1.180 6.6 7.7 recombinant His-tagged enzyme Streptomyces glaucescens