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Literature summary extracted from
- Isolation and function of spinach leaf beta-ketoacyl-[acyl-carrier-protein] synthases (1982), Proc. Natl. Acad. Sci. USA, 79, 5808-5812.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.1.41 | arsenite | synthase II and I | Spinacia oleracea |  |
| 2.3.1.41 | cerulenin | - |
Spinacia oleracea | |
| 2.3.1.41 | N-ethylmaleimide | 5 mM | Spinacia oleracea | |
| 2.3.1.41 | p-chloromercuribenzoate | complete inhibition at 1 mM | Spinacia oleracea | |
| 2.3.1.179 | arsenite | 1 mM, 42% inhibition | Spinacia oleracea | |
| 2.3.1.179 | cerulenin | 0.05 mM, 50% inhibition | Spinacia oleracea | |
| 2.3.1.179 | NEM | 5 mM, complete inhibition | Spinacia oleracea | |
| 2.3.1.179 | PCMB | 1 mM, complete inhibition | Spinacia oleracea | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.41 | 0.0036 | - |
palmitoyl-[acyl-carrier protein] | synthase II | Spinacia oleracea | |
| 2.3.1.41 | 0.0055 | - |
malonyl-CoA | - |
Spinacia oleracea | |
| 2.3.1.41 | 0.0133 | - |
decanoyl-[acyl-carrier protein] | synthase II | Spinacia oleracea | |
| 2.3.1.41 | 0.0139 | - |
tetradecanoyl-[acyl-carrier protein] | synthase II | Spinacia oleracea | |
| 2.3.1.179 | 0.0036 | - |
palmitoyl-[acyl-carrier protein] | - |
Spinacia oleracea | |
| 2.3.1.179 | 0.0055 | - |
malonyl-CoA | - |
Spinacia oleracea | |
| 2.3.1.179 | 0.0133 | - |
decanoyl-[acyl-carrier protein] | - |
Spinacia oleracea | |
| 2.3.1.179 | 0.0139 | - |
myristoyl-[acyl-carrier protein] | - |
Spinacia oleracea | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.41 | 56000 | - |
synthase I, gel filtration | Spinacia oleracea |
| 2.3.1.41 | 57500 | - |
synthase II, gel filtration | Spinacia oleracea |
| 2.3.1.179 | 56000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Spinacia oleracea | part of non-associated fatty acid synthase system of plants and prokaryotes | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Spinacia oleracea | involved in biosynthesis of saturated fatty acids | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.179 | additional information | Spinacia oleracea | the enzyme plays a key role in synthesis of C18 fatty acids | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.1.41 | Spinacia oleracea | - |
synthase I and II | - |
| 2.3.1.179 | Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.1.41 | synthases II | Spinacia oleracea |
| 2.3.1.179 | - |
Spinacia oleracea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.3.1.41 | leaf | - |
Spinacia oleracea | - |
| 2.3.1.179 | leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.41 | additional information | - |
- |
Spinacia oleracea |
| 2.3.1.179 | 0.023 | - |
- |
Spinacia oleracea |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.3.1.41 | -70°C, at least 1 month | Spinacia oleracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.41 | acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | synthase I | Spinacia oleracea | acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | part of non-associated fatty acid synthase system of plants and prokaryotes | Spinacia oleracea | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | involved in biosynthesis of saturated fatty acids | Spinacia oleracea | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | synthase I | Spinacia oleracea | dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | only synthase II, best substrate | Spinacia oleracea | 3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | i.e. palmitoyl-[acyl-carrier-protein] | Spinacia oleracea | 3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | best substrate, synthase I | Spinacia oleracea | 3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | malonyl-CoA + palmitoyl-[acyl-carrier protein] | - |
Spinacia oleracea | ? | - |
? | |
| 2.3.1.41 | additional information | synthase I is specific for short-chain fatty acid prolongation while synthase II is active in C18 formation from C16 | Spinacia oleracea | ? | - |
? | |
| 2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Spinacia oleracea | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | not octodecanoyl-[acyl-carrier-protein], i.e stearoyl-[acyl-carrier-protein] | Spinacia oleracea | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.41 | tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | i.e. myristoyl-[acyl-carrier-protein], only synthase II | Spinacia oleracea | 3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.179 | decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Spinacia oleracea | ? + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.179 | malonyl-CoA + palmitoyl-[acyl-carrier protein] | - |
Spinacia oleracea | ? | - |
? | |
| 2.3.1.179 | additional information | the enzyme plays a key role in synthesis of C18 fatty acids | Spinacia oleracea | ? | - |
? | |
| 2.3.1.179 | additional information | enzyme is inactive with stearoyl-[acyl-carrier-protein] | Spinacia oleracea | ? | - |
? | |
| 2.3.1.179 | myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Spinacia oleracea | ? + CO2 + [acyl-carrier protein] | - |
? | |
| 2.3.1.179 | palmitoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Spinacia oleracea | ? + CO2 + [acyl-carrier protein] | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.3.1.179 | beta-ketoacyl-ACP synthase II | - |
Spinacia oleracea |
| 2.3.1.179 | beta-ketoacyl-[acyl-carrier-protein] synthase II | - |
Spinacia oleracea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.41 | 8.1 | 8.5 | - |
Spinacia oleracea |
| 2.3.1.179 | 8.1 | 8.5 | - |
Spinacia oleracea |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.41 | 6.8 | 9.1 | - |
Spinacia oleracea |
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