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Literature summary extracted from

  • MacKintosh, R.W.; Hardie, D.G.; Slabas, A.R.
    A new assay procedure to study the induction of beta-ketoacyl-ACP synthase I and II, and the complete purification of beta-ketoacyl-ACP synthase I from developing seeds of oilseed rape (Brassica napus) (1989), Biochim. Biophys. Acta, 1002, 114-124.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.1.41 dithiothreitol stabilizes Brassica napus
2.3.1.41 proteinase inhibitors stabilize during purification Brassica napus
2.3.1.41 Triton X-100 facilitates extraction from oil seeds Brassica napus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 cerulenin no inhibition Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 43000
-
 2 * 43000, SDS-PAGE Brassica napus
2.3.1.41 86700
-
 synthase I, gel filtration Brassica napus
2.3.1.41 87400
-
 synthase II, gel filtration Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Brassica napus
-
 synthase I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41
-
 Brassica napus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.41 seed germinating Brassica napus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.41 additional information
-
-
 Brassica napus

Storage Stability

EC Number Storage Stability Organism
2.3.1.41 -70° to -20°C, 90% loss of activity within 7 days Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] i.e. palmitoyl-[acyl-carrier-protein] Brassica napus 3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Brassica napus 3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 octadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase I and II Brassica napus 3-oxoeicosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Brassica napus 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.41 dimer 2 * 43000, SDS-PAGE Brassica napus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.41 37
-
 assay at Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.41 6.3
-
-
 Brassica napus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.41 5.6 7.2 synthase II, about half-maximal activity at pH 5.6 and pH 7.2 Brassica napus
2.3.1.41 5.9 6.8 synthase I, about half-maximal activity at pH 5.9 and pH 6.8 Brassica napus