EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.39 | expressed in Escherichia coli as a hexahistidine-tagged fusion protein | Streptomyces coelicolor |
EC Number | General Stability | Organism |
---|---|---|
2.3.1.39 | enzyme labile during chromatography | Streptomyces coelicolor |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.39 | phenylmethanesulfonyl fluoride | - |
Escherichia coli | |
2.3.1.39 | phenylmethanesulfonyl fluoride | - |
Streptomyces coelicolor |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.39 | 0.06 | - |
malonyl-CoA | hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein | Streptomyces coelicolor | |
2.3.1.39 | 0.073 | - |
acyl-carrier protein | hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein | Streptomyces coelicolor | |
2.3.1.39 | 0.25 | - |
malonyl-CoA | reaction with E. coli acyl-carrier-protein | Escherichia coli | |
2.3.1.39 | 0.327 | - |
malonyl-CoA | hexahistidine-tagged fusion protein, reaction with E. coli acyl-carrier-protein | Streptomyces coelicolor | |
2.3.1.39 | 0.351 | - |
acyl-carrier protein | reaction with E. coli acyl-carrier-protein | Escherichia coli | |
2.3.1.39 | 0.776 | - |
acyl-carrier protein | reaction with E. coli acyl-carrier-protein | Streptomyces coelicolor |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.39 | 34110 | - |
electrospray mass spectrometry, hexahistidine-tagged fusion protein | Streptomyces coelicolor |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.39 | Escherichia coli | - |
- |
- |
2.3.1.39 | Streptomyces coelicolor | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.39 | of the hexahistidine-tagged fusion protein, using Ni(II) affinity chromatography, column chromatography on Q-Sepharose, phenyl Superose and Mono-Q | Streptomyces coelicolor |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.39 | malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] | mechanism, kinetic studies | Streptomyces coelicolor |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.39 | additional information | - |
- |
Escherichia coli |
2.3.1.39 | additional information | - |
hexahistidine-tagged fusion protein | Streptomyces coelicolor |
2.3.1.39 | 780 | - |
hexahistidine-tagged fusion protein, at pH 6.5 and 30°C | Streptomyces coelicolor |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.39 | malonyl-CoA + acyl-carrier protein | - |
Escherichia coli | CoA + malonyl-[acyl-carrier protein] | - |
? | |
2.3.1.39 | malonyl-CoA + acyl-carrier protein | - |
Streptomyces coelicolor | CoA + malonyl-[acyl-carrier protein] | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.39 | 30 | - |
assay at | Escherichia coli |
2.3.1.39 | 30 | - |
assay at | Streptomyces coelicolor |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.39 | 420 | - |
malonyl CoA | hexahistidine-tagged fusion protein, reaction with E. coli acyl-carrier-protein | Streptomyces coelicolor | |
2.3.1.39 | 450 | - |
malonyl CoA | hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein | Streptomyces coelicolor | |
2.3.1.39 | 1580 | - |
malonyl CoA | reaction with E. coli acyl-carrier-protein | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.39 | 6.5 | - |
- |
Escherichia coli |
2.3.1.39 | 6.5 | - |
assay at | Streptomyces coelicolor |