Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stapleton, S.R.; Jaworski, J.G.
    Characterization and purification of malonyl-coenzyme A:[acyl-carrier protein] transacylases from spinach and Anabaena variabilis (1984), Biochim. Biophys. Acta, 794, 240-248.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.39 CoA kinetics Spinacia oleracea
2.3.1.39 CoA kinetics Trichormus variabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.39 0.3
-
 malonyl-CoA sequential mechanism Trichormus variabilis
2.3.1.39 0.4
-
 malonyl-CoA ping-pong mechanism Trichormus variabilis
2.3.1.39 0.4
-
 acyl-carrier protein sequential mechanism Spinacia oleracea
2.3.1.39 0.4
-
 acyl-carrier protein sequential mechanism Trichormus variabilis
2.3.1.39 0.5
-
 malonyl-CoA sequential mechanism Spinacia oleracea
2.3.1.39 0.6
-
 acyl-carrier protein ping-pong mechanism Trichormus variabilis
2.3.1.39 1.3
-
 acyl-carrier protein ping-pong mechanism Spinacia oleracea
2.3.1.39 1.4
-
 malonyl-CoA ping-pong mechanism Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.39 31000
-
 HPLC gel filtration Spinacia oleracea
2.3.1.39 36000
-
 HPLC gel filtration Trichormus variabilis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Spinacia oleracea
-
 spinach
-
2.3.1.39 Trichormus variabilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 ammonium sulfate fractionation and DEAE-cellulose Spinacia oleracea
2.3.1.39 ammonium sulfate fractionation and DEAE-cellulose Trichormus variabilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] mechanism, kinetic studies Spinacia oleracea
a
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] mechanism, kinetic studies Trichormus variabilis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.39 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.39 0.0683
-
 purified enzyme Spinacia oleracea
2.3.1.39 0.1541
-
 purified enzyme Trichormus variabilis

Storage Stability

EC Number Storage Stability Organism
2.3.1.39 -20°C, frozen in liquid nitrogen, several months Spinacia oleracea
2.3.1.39 -20°C, frozen in liquid nitrogen, several months Trichormus variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Spinacia oleracea CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Trichormus variabilis CoA + malonyl-[acyl-carrier protein]
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.39 25
-
 assay at Spinacia oleracea
2.3.1.39 25
-
 assay at Trichormus variabilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.39 80
-
 inactivation after 20 min Spinacia oleracea
2.3.1.39 80
-
 inactivation after 20 min Trichormus variabilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 8
-
 higher activity in Tris buffer than in potassium phosphate buffer Spinacia oleracea
2.3.1.39 8.5
-
 higher activity in Tris buffer than in potassium phosphate buffer Trichormus variabilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.39 additional information
-
 additional information
-
 Spinacia oleracea
2.3.1.39 additional information
-
 additional information
-
 Trichormus variabilis
2.3.1.39 0.009
-
 CoA with respect to acyl carrier protein Trichormus variabilis
2.3.1.39 0.015
-
 CoA with respect to malonyl-CoA Trichormus variabilis
2.3.1.39 0.023
-
 CoA with respect to malonyl-CoA Spinacia oleracea
2.3.1.39 0.03
-
 CoA with respect to acyl carrier protein Spinacia oleracea