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Literature summary extracted from

  • Rangan, V.S.; Joshi, A.K.; Smith, S.
    Fatty acid synthase dimers containing catalytically active beta-ketoacyl synthase or malonyl/acetyltransferase domains in only one subunit can support fatty acid synthesis at the acyl carrier protein domains of both subunits (1998), J. Biol. Chem., 273, 34949-34953.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.38 expression of wild-type and His-tagged mutant S581A as His-tagged proteins in Escherichia coli and as FLAG-tagged proteins in Sf9 insect cells via baculovirus infection Rattus norvegicus
2.3.1.85
-
 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.38 S581A nearly no remaining activity, below 0.03% compared to wild-type Rattus norvegicus
2.3.1.85 C161A no beta-ketoacyl synthase activity Rattus norvegicus
2.3.1.85 K326A no beta-ketoacyl synthase activity Rattus norvegicus
2.3.1.85 S518A no malonyl/acetyltransferase activity Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.38 Rattus norvegicus
-
-
-
2.3.1.85 Rattus norvegicus
-
 wild-type and mutant
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.38 additional information
-
-
 Rattus norvegicus
2.3.1.38 3.22
-
 recombinant purified wild-type enzyme, carboxyterminally FLAG-tagged Rattus norvegicus
2.3.1.38 3.81
-
 recombinant purified wild-type enzyme, carboxyterminally His-tagged Rattus norvegicus
2.3.1.85 additional information
-
 specific activities of subdomains Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.38 acetyl-CoA + pantetheine dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase Rattus norvegicus CoA + acetyl-S-pantetheine
-
 r
2.3.1.38 acetyl-CoA + [acyl-carrier protein] dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase Rattus norvegicus CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 additional information enzyme is part of fatty acid synthetase complex, consisting of 2 subunits Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.38 malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase i.e. MAT Rattus norvegicus