Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rangan, V.S.; Smith, S.
    Alteration of the substrate specificity of the malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase domain of the multifunctional fatty acid synthase by mutation of a single arginine residue (1997), J. Biol. Chem., 272, 11975-11978.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.38 expression of wild-type and mutants in Escherichia coli BL21 (DE3) Rattus norvegicus
2.3.1.39 expression of mutant proteins R606A and R606K in Escherichia coli Rattus norvegicus
2.3.1.85 transacyclase domain Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.38 R606A oligonucleotide-directed in vitro mutagenesis, 6.6fold increase in activity about 16000fold increase in selectivity for acetyl-CoA against malonyl-CoA, increased production of medium-chain length fatty acids Rattus norvegicus
2.3.1.38 R606K oligonucleotide-directed in vitro mutagenesis, 1.7fold increase in activity about 16fold increase in selectivity for acetyl-CoA against malonyl-CoA Rattus norvegicus
2.3.1.85 R606A reduced malonyl/acetyltransferase activity, increased transacylase activity, 16000fold increased selectivity for acetyl-CoA, 8.5fold increase of Km for malonyl-CoA Rattus norvegicus
2.3.1.85 R606K reduced malonyl/acetyltransferase activity, increased transacylase activity, 16fold increased selectivity for acetyl-CoA Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.38 0.0018
-
 acetyl-CoA recombinant purified mutant R606A Rattus norvegicus
2.3.1.38 0.0029
-
 acetyl-CoA recombinant purified mutant R606K Rattus norvegicus
2.3.1.38 0.0039
-
 acetyl-CoA recombinant purified wild-type enzyme Rattus norvegicus
2.3.1.39 0.0013
-
 malonyl-CoA R606K mutant Rattus norvegicus
2.3.1.39 0.0019
-
 malonyl-CoA wild-type Rattus norvegicus
2.3.1.39 0.0162
-
 malonyl-CoA R606A mutant Rattus norvegicus
2.3.1.85 0.0013
-
 malonyl-CoA R606K mutant Rattus norvegicus
2.3.1.85 0.0018
-
 acetyl-CoA R606A mutant Rattus norvegicus
2.3.1.85 0.0019
-
 malonyl-CoA wild-type enzyme Rattus norvegicus
2.3.1.85 0.0029
-
 acetyl-CoA R606K mutant Rattus norvegicus
2.3.1.85 0.0039
-
 acetyl-CoA wild-type enzyme Rattus norvegicus
2.3.1.85 0.0162
-
 malonyl-CoA R606A mutant Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.39 42000
-
 mutant proteins R606A and R606K, Western analysis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.38 Rattus norvegicus
-
-
-
2.3.1.39 Rattus norvegicus
-
 malonyl transferase domain of EC 2.3.1.85
-
2.3.1.85 Rattus norvegicus
-
 wild-type and mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.38 recombinant mutants from E. coli Rattus norvegicus
2.3.1.39 of the mutants R606A und R606K Rattus norvegicus
2.3.1.85 95% pure, transacyclase domain Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.38 acetyl-CoA + an [acyl-carrier protein] = CoA + an acetyl-[acyl-carrier protein] mechanism Rattus norvegicus
a
2.3.1.38 acetyl-CoA + an [acyl-carrier protein] = CoA + an acetyl-[acyl-carrier protein] Arg-606 is responsible for malonyl-CoA specificity of the bifunctional enzyme Rattus norvegicus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.38 recombinant enzyme purified from E. coli, refolding after treatment of inclusion bodies with urea Rattus norvegicus
2.3.1.39 of the mutant proteins R606A and R606K Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.38 additional information
-
-
 Rattus norvegicus
2.3.1.38 2.68
-
 recombinant purified wild-type enzyme Rattus norvegicus
2.3.1.38 4.53
-
 recombinant purified mutant R606K Rattus norvegicus
2.3.1.38 17.8
-
 recombinant purified mutant R606A Rattus norvegicus
2.3.1.39 0.015
-
 R606A mutant Rattus norvegicus
2.3.1.39 0.154
-
 R606K mutant Rattus norvegicus
2.3.1.39 1.61
-
 wild-type Rattus norvegicus
2.3.1.85 0.015
-
 for malonyl-CoA, R606A mutant Rattus norvegicus
2.3.1.85 0.154
-
 for malonyl-CoA, R606K mutant Rattus norvegicus
2.3.1.85 1.61
-
 for malonyl-CoA, wild-type enzyme Rattus norvegicus
2.3.1.85 2.68
-
 for acetyl-CoA, wild-type enzyme Rattus norvegicus
2.3.1.85 4.53
-
 for acetyl-CoA, R606K mutant Rattus norvegicus
2.3.1.85 17.8
-
 for acetyl-CoA, R606A mutant Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.38 acetyl-CoA + pantetheine dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase Rattus norvegicus CoA + acetyl-S-pantetheine
-
 r
2.3.1.38 acetyl-CoA + pantetheine octanoyl-S-pantetheine, decanoyl-S-pantetheine, and dodecanoyl-S-pantetheine can also serve as substrates for the backward reaction Rattus norvegicus CoA + acetyl-S-pantetheine
-
 r
2.3.1.38 acetyl-CoA + [acyl-carrier protein] dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase Rattus norvegicus CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + pantetheine reaction catalysed by the malonyl transferase domain of EC 2.3.1.85 Rattus norvegicus malonyl-pantetheine + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.38 malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase
-
 Rattus norvegicus
2.3.1.38 More cf. EC 2.3.1.85 and EC 2.3.1.86 Rattus norvegicus
2.3.1.38 More dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase Rattus norvegicus
2.3.1.39 More in vertebrates, yeast and mycobacteria malonyl transferase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.38 1.9
-
 acetyl-CoA recombinant purified wild-type enzyme Rattus norvegicus
2.3.1.38 3.4
-
 acetyl-CoA recombinant purified mutant R606K Rattus norvegicus
2.3.1.38 4.2
-
 acetyl-CoA recombinant purified mutant R606A Rattus norvegicus
2.3.1.39 0.01
-
 malonyl CoA R606A mutant Rattus norvegicus
2.3.1.39 0.12
-
 malonyl CoA R606K mutant Rattus norvegicus
2.3.1.39 1.2
-
 malonyl CoA wild-type Rattus norvegicus
2.3.1.85 0.0002
-
 malonyl-CoA R606K mutant Rattus norvegicus
2.3.1.85 0.00167
-
 malonyl-CoA R606A mutant Rattus norvegicus
2.3.1.85 0.02
-
 malonyl-CoA wild-type enzyme Rattus norvegicus
2.3.1.85 0.0317
-
 acetyl-CoA wild-type enzyme Rattus norvegicus
2.3.1.85 0.0567
-
 acetyl-CoA R606K mutant Rattus norvegicus
2.3.1.85 0.237
-
 acetyl-CoA R606A mutant Rattus norvegicus