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Literature summary extracted from

  • Williamson, I.P.; Wakil, S.J.
    Studies on the mechanism of fatty acid synthesis. XVII. Preparation and general properties of acetyl coenzyme A and malonyl coenzyme A-acyl carrier protein transacylases (1966), J. Biol. Chem., 241, 2326-2332.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.38 EDTA at 5 mM stabilize Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.38 iodoacetamide
-
 Escherichia coli
2.3.1.38 N-ethylmaleimide
-
 Escherichia coli
2.3.1.39 iodoacetamide
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein Escherichia coli initial reaction in de novo fatty acid synthesis, part of non-associated fatty acid synthase system of plants and prokaryotes CoA + malonyl-acyl-carrier protein
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.38 Escherichia coli
-
-
-
2.3.1.38 Escherichia coli E26
-
-
-
2.3.1.39 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.38
-
 Escherichia coli
2.3.1.39
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.38 0.059
-
-
 Escherichia coli
2.3.1.39 2.3
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.38 -10°C, unpredictable instability on storage, sometimes total loss of activity occured immediately after thawing Escherichia coli
2.3.1.38 0°C, inactivation within several days Escherichia coli
2.3.1.39 -10°C, several weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.38 acetyl-CoA + [acyl-carrier protein] via acetyl-enzyme intermediate Escherichia coli CoA + acetyl-[acyl-carrier protein]
-
 r
2.3.1.38 acetyl-CoA + [acyl-carrier protein] via acetyl-enzyme intermediate Escherichia coli E26 CoA + acetyl-[acyl-carrier protein]
-
 r
2.3.1.38 butyryl-CoA + [acyl-carrier protein] 9.8% activity of that with acetyl-CoA Escherichia coli CoA + butyryl-[acyl-carrier protein]
-
 ?
2.3.1.38 butyryl-CoA + [acyl-carrier protein] 9.8% activity of that with acetyl-CoA Escherichia coli E26 CoA + butyryl-[acyl-carrier protein]
-
 ?
2.3.1.38 hexanoyl-CoA + [acyl-carrier protein] 4.6% activity of that with acetyl-CoA Escherichia coli CoA + hexanoyl-[acyl-carrier protein]
-
 ?
2.3.1.38 hexanoyl-CoA + [acyl-carrier protein] 4.6% activity of that with acetyl-CoA Escherichia coli E26 CoA + hexanoyl-[acyl-carrier protein]
-
 ?
2.3.1.38 octanoyl-CoA + [acyl-carrier protein] 10.3% activity of that with acetyl-CoA Escherichia coli CoA + octanoyl-[acyl-carrier protein]
-
 ?
2.3.1.38 octanoyl-CoA + [acyl-carrier protein] 10.3% activity of that with acetyl-CoA Escherichia coli E26 CoA + octanoyl-[acyl-carrier protein]
-
 ?
2.3.1.38 propionyl-CoA + [acyl-carrier protein] transacylation at 23.4% the rate of acetyl-CoA Escherichia coli CoA + propionyl-[acyl-carrier protein]
-
 ?
2.3.1.38 propionyl-CoA + [acyl-carrier protein] transacylation at 23.4% the rate of acetyl-CoA Escherichia coli E26 CoA + propionyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein
-
 Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
 r
2.3.1.39 malonyl-CoA + acyl-carrier protein initial reaction in de novo fatty acid synthesis, part of non-associated fatty acid synthase system of plants and prokaryotes Escherichia coli CoA + malonyl-acyl-carrier protein
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.38 38
-
 assay at Escherichia coli
2.3.1.39 38
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.38 40
-
 30% loss of activity after 40 min Escherichia coli
2.3.1.38 50
-
 t1/2: 18 min Escherichia coli
2.3.1.38 60
-
 t1/2: 9 min Escherichia coli
2.3.1.38 100
-
 inactivation after 1 min Escherichia coli
2.3.1.39 50
-
 1 h stable Escherichia coli
2.3.1.39 60
-
 stable for at least 40 min Escherichia coli
2.3.1.39 80
-
 20% loss of activity after 20 min Escherichia coli
2.3.1.39 100
-
 t1/2: 15 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.38 6
-
-
 Escherichia coli
2.3.1.39 6 7
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.38 5 8.5 about 60% of maximal activity at pH 5.0 and pH 8.5 Escherichia coli