BRENDA - Enzyme Database show

Role of arginine 439 in substrate binding of 5-aminolevulinate synthase

Tan, D.; Harrison, T.; Hunter, G.A.; Ferreira, G.C.; Biochemistry 37, 1478-1484 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.37
overexpression of His-tagged wild-type and mutants in Escherichia coli
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.37
R433K
active site mutant, 2fold increased activity
Mus musculus
2.3.1.37
R433L
active site mutant, similar to the wild-type
Mus musculus
2.3.1.37
R439K
active site mutant, 77% activity compared to the wild-type, 9-13fold increased Km for both substrates, 5fold increased dissociation constant for glycine
Mus musculus
2.3.1.37
R439L
active site mutant, no activity, 30fold increased dissociation constant for glycine
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.002
-
succinyl-CoA
recombinant erythroid isoform wild-type
Mus musculus
2.3.1.37
0.0022
-
succinyl-CoA
recombinant erythroid isoform mutant R433K
Mus musculus
2.3.1.37
0.0032
-
succinyl-CoA
recombinant erythroid isoform mutant R433L
Mus musculus
2.3.1.37
0.027
-
succinyl-CoA
recombinant erythroid isoform mutant R439K
Mus musculus
2.3.1.37
11.7
-
glycine
recombinant erythroid isoform
Mus musculus
2.3.1.37
14.4
-
glycine
recombinant erythroid isoform mutant R433K
Mus musculus
2.3.1.37
18.4
-
glycine
recombinant erythroid isoform mutant R433L
Mus musculus
2.3.1.37
103
-
glycine
recombinant erythroid isoform mutant R439K
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Mus musculus
-
erythroid-specific isoform
-
Reaction
EC Number
Reaction
Commentary
Organism
2.3.1.37
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2
Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486857
Mus musculus
5-aminolevulinate + CoA + CO2
-
486857
Mus musculus
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.508
-
succinyl-CoA
recombinant erythroid mutant R439K
Mus musculus
2.3.1.37
0.658
-
succinyl-CoA
recombinant erythroid isoform
Mus musculus
2.3.1.37
0.842
-
succinyl-CoA
recombinant erythroid mutant R433L
Mus musculus
2.3.1.37
1.35
-
succinyl-CoA
recombinant erythroid mutant R433K
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
overexpression of His-tagged wild-type and mutants in Escherichia coli
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.37
R433K
active site mutant, 2fold increased activity
Mus musculus
2.3.1.37
R433L
active site mutant, similar to the wild-type
Mus musculus
2.3.1.37
R439K
active site mutant, 77% activity compared to the wild-type, 9-13fold increased Km for both substrates, 5fold increased dissociation constant for glycine
Mus musculus
2.3.1.37
R439L
active site mutant, no activity, 30fold increased dissociation constant for glycine
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.002
-
succinyl-CoA
recombinant erythroid isoform wild-type
Mus musculus
2.3.1.37
0.0022
-
succinyl-CoA
recombinant erythroid isoform mutant R433K
Mus musculus
2.3.1.37
0.0032
-
succinyl-CoA
recombinant erythroid isoform mutant R433L
Mus musculus
2.3.1.37
0.027
-
succinyl-CoA
recombinant erythroid isoform mutant R439K
Mus musculus
2.3.1.37
11.7
-
glycine
recombinant erythroid isoform
Mus musculus
2.3.1.37
14.4
-
glycine
recombinant erythroid isoform mutant R433K
Mus musculus
2.3.1.37
18.4
-
glycine
recombinant erythroid isoform mutant R433L
Mus musculus
2.3.1.37
103
-
glycine
recombinant erythroid isoform mutant R439K
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486857
Mus musculus
5-aminolevulinate + CoA + CO2
-
486857
Mus musculus
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.508
-
succinyl-CoA
recombinant erythroid mutant R439K
Mus musculus
2.3.1.37
0.658
-
succinyl-CoA
recombinant erythroid isoform
Mus musculus
2.3.1.37
0.842
-
succinyl-CoA
recombinant erythroid mutant R433L
Mus musculus
2.3.1.37
1.35
-
succinyl-CoA
recombinant erythroid mutant R433K
Mus musculus