Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gong, J.; Hunter, G.A.; Ferreira, G.C.
    Aspartate-279 in aminolevulinate synthase affects enzyme catalysis through enhancing the function of the pyridoxal 5'-phosphate cofactor (1998), Biochemistry, 37, 3509-3517.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.37 expression of erythroid-specific isoform ALAS-E and mutants in Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.37 D279A exchange mutant of potential cofactor binding residue Asp279, no activity, dissociation constant for pyridoxal 5'-phosphate is 19fold increased, different mode of cofactor binding, no formation of quinonoid reaction intermediate, which can be restored by addition of analogue N-methyl-pyridoxal 5'-phosphate Mus musculus
2.3.1.37 D279E exchange mutant of potential cofactor binding residue Asp279, 30fold reduced catalytic efficiency for succinyl-CoA compared to the wild-type Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.37 0.002
-
 succinyl-CoA recombinant erythroid isoform wild-type Mus musculus
2.3.1.37 0.035
-
 succinyl-CoA recombinant erythroid isoform mutant D279E Mus musculus
2.3.1.37 23
-
 glycine recombinant erythroid isoform wild-type Mus musculus
2.3.1.37 140
-
 glycine recombinant erythroid isoform mutant D279E Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Mus musculus
-
 erythroid-specific isoform
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37 recombinant wild-type and mutants from E. coli Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.37 succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation Mus musculus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.37 0.0967
-
 succinyl-CoA recombinant erythroid isoform mutant D279E Mus musculus
2.3.1.37 0.167
-
 succinyl-CoA recombinant erythroid isoform Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 N-methyl-pyridoxal 5'-phosphate in vitro, mutant D279A Mus musculus
2.3.1.37 pyridoxal 5'-phosphate required Mus musculus