BRENDA - Enzyme Database show

Aspartate-279 in aminolevulinate synthase affects enzyme catalysis through enhancing the function of the pyridoxal 5'-phosphate cofactor

Gong, J.; Hunter, G.A.; Ferreira, G.C.; Biochemistry 37, 3509-3517 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.37
expression of erythroid-specific isoform ALAS-E and mutants in Escherichia coli
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.37
D279A
exchange mutant of potential cofactor binding residue Asp279, no activity, dissociation constant for pyridoxal 5'-phosphate is 19fold increased, different mode of cofactor binding, no formation of quinonoid reaction intermediate, which can be restored by addition of analogue N-methyl-pyridoxal 5'-phosphate
Mus musculus
2.3.1.37
D279E
exchange mutant of potential cofactor binding residue Asp279, 30fold reduced catalytic efficiency for succinyl-CoA compared to the wild-type
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.002
-
succinyl-CoA
recombinant erythroid isoform wild-type
Mus musculus
2.3.1.37
0.035
-
succinyl-CoA
recombinant erythroid isoform mutant D279E
Mus musculus
2.3.1.37
23
-
glycine
recombinant erythroid isoform wild-type
Mus musculus
2.3.1.37
140
-
glycine
recombinant erythroid isoform mutant D279E
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Mus musculus
-
erythroid-specific isoform
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
recombinant wild-type and mutants from E. coli
Mus musculus
Reaction
EC Number
Reaction
Commentary
Organism
2.3.1.37
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2
Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486856
Mus musculus
5-aminolevulinate + CoA + CO2
-
486856
Mus musculus
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.0967
-
succinyl-CoA
recombinant erythroid isoform mutant D279E
Mus musculus
2.3.1.37
0.167
-
succinyl-CoA
recombinant erythroid isoform
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
N-methyl-pyridoxal 5'-phosphate
in vitro, mutant D279A
Mus musculus
2.3.1.37
pyridoxal 5'-phosphate
required
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
expression of erythroid-specific isoform ALAS-E and mutants in Escherichia coli
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
N-methyl-pyridoxal 5'-phosphate
in vitro, mutant D279A
Mus musculus
2.3.1.37
pyridoxal 5'-phosphate
required
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.37
D279A
exchange mutant of potential cofactor binding residue Asp279, no activity, dissociation constant for pyridoxal 5'-phosphate is 19fold increased, different mode of cofactor binding, no formation of quinonoid reaction intermediate, which can be restored by addition of analogue N-methyl-pyridoxal 5'-phosphate
Mus musculus
2.3.1.37
D279E
exchange mutant of potential cofactor binding residue Asp279, 30fold reduced catalytic efficiency for succinyl-CoA compared to the wild-type
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.002
-
succinyl-CoA
recombinant erythroid isoform wild-type
Mus musculus
2.3.1.37
0.035
-
succinyl-CoA
recombinant erythroid isoform mutant D279E
Mus musculus
2.3.1.37
23
-
glycine
recombinant erythroid isoform wild-type
Mus musculus
2.3.1.37
140
-
glycine
recombinant erythroid isoform mutant D279E
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
recombinant wild-type and mutants from E. coli
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486856
Mus musculus
5-aminolevulinate + CoA + CO2
-
486856
Mus musculus
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.0967
-
succinyl-CoA
recombinant erythroid isoform mutant D279E
Mus musculus
2.3.1.37
0.167
-
succinyl-CoA
recombinant erythroid isoform
Mus musculus