BRENDA - Enzyme Database show

The role of tyrosine 121 in cofactor binding of 5-aminolevulinate synthase

Tan, D.; Barber, M.J.; Ferreira, G.C.; Protein Sci. 7, 1208-1213 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.37
overexpression of His-tagged wild-type and mutants in Escherichia coli
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.37
Y121F
exchange mutant of potential cofactor binding residue Tyr121, 5% activity compared to the wild-type, Km for glycine is 5fold increased, lower affinity for pyridoxal 5'phosphate
Mus musculus
2.3.1.37
Y121H
exchange mutant of potential cofactor binding residue Tyr121, 36% activity compared to the wild-type, Km for glycine is 34fold increased, lower affinity for pyridoxal 5'phosphate
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.0016
-
succinyl-CoA
recombinant mutant Y121H
Mus musculus
2.3.1.37
0.002
-
succinyl-CoA
recombinant erythroid isoform wild-type
Mus musculus
2.3.1.37
0.014
-
succinyl-CoA
recombinant mutant Y121F
Mus musculus
2.3.1.37
11.7
-
glycine
recombinant erythroid isoform
Mus musculus
2.3.1.37
52.2
-
glycine
recombinant mutant Y121H
Mus musculus
2.3.1.37
400
-
glycine
recombinant mutant Y121F
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
additional information
-
amino acid sequence alignment
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Mus musculus
-
erythroid-specific isoform
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
recombinant of His-tagged wild-type and mutants from E. coli
Mus musculus
Reaction
EC Number
Reaction
Commentary
Organism
2.3.1.37
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2
Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486855
Mus musculus
5-aminolevulinate + CoA + CO2
-
486855
Mus musculus
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.0355
-
succinyl-CoA
recombinant erythroid isoform mutant Y121H
Mus musculus
2.3.1.37
0.235
-
succinyl-CoA
recombinant erythroid isoform mutant Y121F
Mus musculus
2.3.1.37
0.658
-
succinyl-CoA
recombinant erythroid isoform
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.5
-
-
Mus musculus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.37
6.5
9.5
-
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
overexpression of His-tagged wild-type and mutants in Escherichia coli
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.37
Y121F
exchange mutant of potential cofactor binding residue Tyr121, 5% activity compared to the wild-type, Km for glycine is 5fold increased, lower affinity for pyridoxal 5'phosphate
Mus musculus
2.3.1.37
Y121H
exchange mutant of potential cofactor binding residue Tyr121, 36% activity compared to the wild-type, Km for glycine is 34fold increased, lower affinity for pyridoxal 5'phosphate
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.0016
-
succinyl-CoA
recombinant mutant Y121H
Mus musculus
2.3.1.37
0.002
-
succinyl-CoA
recombinant erythroid isoform wild-type
Mus musculus
2.3.1.37
0.014
-
succinyl-CoA
recombinant mutant Y121F
Mus musculus
2.3.1.37
11.7
-
glycine
recombinant erythroid isoform
Mus musculus
2.3.1.37
52.2
-
glycine
recombinant mutant Y121H
Mus musculus
2.3.1.37
400
-
glycine
recombinant mutant Y121F
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
additional information
-
amino acid sequence alignment
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
recombinant of His-tagged wild-type and mutants from E. coli
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486855
Mus musculus
5-aminolevulinate + CoA + CO2
-
486855
Mus musculus
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.0355
-
succinyl-CoA
recombinant erythroid isoform mutant Y121H
Mus musculus
2.3.1.37
0.235
-
succinyl-CoA
recombinant erythroid isoform mutant Y121F
Mus musculus
2.3.1.37
0.658
-
succinyl-CoA
recombinant erythroid isoform
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.5
-
-
Mus musculus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.37
6.5
9.5
-
Mus musculus