BRENDA - Enzyme Database show

Expression and purification of mammalian 5-aminolevulinate synthase

Dailey, H.A.; Dailey, T.A.; Methods Enzymol. 281, 336-340 (1997)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.3.1.37
short half-life
Gallus gallus
2.3.1.37
short half-life
Mus musculus
2.3.1.37
short half-life
Homo sapiens
2.3.1.37
short half-life
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
protoheme
feed-back inhibition, non-specific isoform ALAS-1
Gallus gallus
2.3.1.37
protoheme
feed-back inhibition, non-specific isoform ALAS-1
Homo sapiens
2.3.1.37
protoheme
feed-back inhibition, non-specific isoform ALAS-1
Mus musculus
2.3.1.37
protoheme
feed-back inhibition, non-specific isoform ALAS-1
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.055
-
succinyl-CoA
-
Mus musculus
2.3.1.37
51
-
glycine
-
Mus musculus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
Iron
regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE
Gallus gallus
2.3.1.37
Iron
regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE
Homo sapiens
2.3.1.37
Iron
regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE
Mus musculus
2.3.1.37
Iron
regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
56000
-
2 * 56000, SDS-PAGE
Mus musculus
2.3.1.37
112000
-
gel filtration
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
additional information
Homo sapiens
enzyme deficiency causes X-linked sideroblastic anemia
?
-
-
-
2.3.1.37
succinyl-CoA + glycine
Gallus gallus
key enzyme in tetrapyrrole biosynthesis
5-aminolevulinate + CoA + CO2
-
Gallus gallus
?
2.3.1.37
succinyl-CoA + glycine
Mus musculus
key enzyme in tetrapyrrole biosynthesis
5-aminolevulinate + CoA + CO2
-
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
Homo sapiens
key enzyme in tetrapyrrole biosynthesis
5-aminolevulinate + CoA + CO2
-
Homo sapiens
?
2.3.1.37
succinyl-CoA + glycine
Rattus norvegicus
key enzyme in tetrapyrrole biosynthesis
5-aminolevulinate + CoA + CO2
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Gallus gallus
-
2 isoforms ALAS-1 and ALAS-2, a houskeeping form and an erythroid-specific form
-
2.3.1.37
Homo sapiens
-
2 isoforms ALAS-1 and ALAS-2, a houskeeping form and an erythroid-specific form
-
2.3.1.37
Mus musculus
-
2 isoforms ALAS-1 and ALAS-2, a houskeeping form and an erythroid-specific form
-
2.3.1.37
Rattus norvegicus
-
non-specific, housekeeping isoform ALAS-N and erythroid-specific isoform ALAS-E
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
recombinant from E. coli
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
additional information
enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence
Gallus gallus
-
2.3.1.37
additional information
enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence
Homo sapiens
-
2.3.1.37
additional information
enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence
Mus musculus
-
2.3.1.37
additional information
enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
additional information
enzyme deficiency causes X-linked sideroblastic anemia
486853
Homo sapiens
?
-
-
-
-
2.3.1.37
succinyl-CoA + glycine
-
486853
Gallus gallus
5-aminolevulinate + CoA + CO2
-
486853
Gallus gallus
?
2.3.1.37
succinyl-CoA + glycine
-
486853
Mus musculus
5-aminolevulinate + CoA + CO2
-
486853
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
-
486853
Homo sapiens
5-aminolevulinate + CoA + CO2
-
486853
Homo sapiens
?
2.3.1.37
succinyl-CoA + glycine
-
486853
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486853
Rattus norvegicus
?
2.3.1.37
succinyl-CoA + glycine
key enzyme in tetrapyrrole biosynthesis
486853
Gallus gallus
5-aminolevulinate + CoA + CO2
-
486853
Gallus gallus
?
2.3.1.37
succinyl-CoA + glycine
key enzyme in tetrapyrrole biosynthesis
486853
Mus musculus
5-aminolevulinate + CoA + CO2
-
486853
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
key enzyme in tetrapyrrole biosynthesis
486853
Homo sapiens
5-aminolevulinate + CoA + CO2
-
486853
Homo sapiens
?
2.3.1.37
succinyl-CoA + glycine
key enzyme in tetrapyrrole biosynthesis
486853
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486853
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
-
Gallus gallus
2.3.1.37
dimer
2 * 56000, SDS-PAGE
Mus musculus
2.3.1.37
dimer
-
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.0095
-
succinyl-CoA
-
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Gallus gallus
2.3.1.37
pyridoxal 5'-phosphate
required
Homo sapiens
2.3.1.37
pyridoxal 5'-phosphate
noncovalently bound; required
Mus musculus
2.3.1.37
pyridoxal 5'-phosphate
required
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Gallus gallus
2.3.1.37
pyridoxal 5'-phosphate
required
Homo sapiens
2.3.1.37
pyridoxal 5'-phosphate
noncovalently bound; required
Mus musculus
2.3.1.37
pyridoxal 5'-phosphate
required
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.37
short half-life
Gallus gallus
2.3.1.37
short half-life
Mus musculus
2.3.1.37
short half-life
Homo sapiens
2.3.1.37
short half-life
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
protoheme
feed-back inhibition, non-specific isoform ALAS-1
Gallus gallus
2.3.1.37
protoheme
feed-back inhibition, non-specific isoform ALAS-1
Homo sapiens
2.3.1.37
protoheme
feed-back inhibition, non-specific isoform ALAS-1
Mus musculus
2.3.1.37
protoheme
feed-back inhibition, non-specific isoform ALAS-1
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.055
-
succinyl-CoA
-
Mus musculus
2.3.1.37
51
-
glycine
-
Mus musculus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
Iron
regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE
Gallus gallus
2.3.1.37
Iron
regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE
Homo sapiens
2.3.1.37
Iron
regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE
Mus musculus
2.3.1.37
Iron
regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
56000
-
2 * 56000, SDS-PAGE
Mus musculus
2.3.1.37
112000
-
gel filtration
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
additional information
Homo sapiens
enzyme deficiency causes X-linked sideroblastic anemia
?
-
-
-
2.3.1.37
succinyl-CoA + glycine
Gallus gallus
key enzyme in tetrapyrrole biosynthesis
5-aminolevulinate + CoA + CO2
-
Gallus gallus
?
2.3.1.37
succinyl-CoA + glycine
Mus musculus
key enzyme in tetrapyrrole biosynthesis
5-aminolevulinate + CoA + CO2
-
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
Homo sapiens
key enzyme in tetrapyrrole biosynthesis
5-aminolevulinate + CoA + CO2
-
Homo sapiens
?
2.3.1.37
succinyl-CoA + glycine
Rattus norvegicus
key enzyme in tetrapyrrole biosynthesis
5-aminolevulinate + CoA + CO2
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
recombinant from E. coli
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
additional information
enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence
Gallus gallus
-
2.3.1.37
additional information
enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence
Homo sapiens
-
2.3.1.37
additional information
enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence
Mus musculus
-
2.3.1.37
additional information
enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
additional information
enzyme deficiency causes X-linked sideroblastic anemia
486853
Homo sapiens
?
-
-
-
-
2.3.1.37
succinyl-CoA + glycine
-
486853
Gallus gallus
5-aminolevulinate + CoA + CO2
-
486853
Gallus gallus
?
2.3.1.37
succinyl-CoA + glycine
-
486853
Mus musculus
5-aminolevulinate + CoA + CO2
-
486853
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
-
486853
Homo sapiens
5-aminolevulinate + CoA + CO2
-
486853
Homo sapiens
?
2.3.1.37
succinyl-CoA + glycine
-
486853
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486853
Rattus norvegicus
?
2.3.1.37
succinyl-CoA + glycine
key enzyme in tetrapyrrole biosynthesis
486853
Gallus gallus
5-aminolevulinate + CoA + CO2
-
486853
Gallus gallus
?
2.3.1.37
succinyl-CoA + glycine
key enzyme in tetrapyrrole biosynthesis
486853
Mus musculus
5-aminolevulinate + CoA + CO2
-
486853
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
key enzyme in tetrapyrrole biosynthesis
486853
Homo sapiens
5-aminolevulinate + CoA + CO2
-
486853
Homo sapiens
?
2.3.1.37
succinyl-CoA + glycine
key enzyme in tetrapyrrole biosynthesis
486853
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486853
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
-
Gallus gallus
2.3.1.37
dimer
2 * 56000, SDS-PAGE
Mus musculus
2.3.1.37
dimer
-
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.0095
-
succinyl-CoA
-
Mus musculus