Literature summary extracted from
Ferreira, G.C.; Vajapey, U.; Hafez, O.; Hunter, G.A.; Barber, M.J.
Aminolevulinate synthase: lysine 313 is not essential for binding the pyridoxal phosphate cofactor but is essential for catalysis (1995), Protein Sci., 4, 1001-1006.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.3.1.37 |
expression of erythroid-specific isoform ALAS-E and mutants in Escherichia coli |
Mus musculus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.3.1.37 |
K313A |
site-directed mutagenesis, mutants of erythroid-specific isoform, exchange of active site lysine residue 313, binding of pyridoxal 5'-phosphate and glycine noncovalently, reduced activity, because covalent binding is required |
Mus musculus |
2.3.1.37 |
K313G |
site-directed mutagenesis, mutants of erythroid-specific isoform, exchange of active site lysine residue 313, binding of pyridoxal 5'-phosphate and glycine noncovalently, reduced activity, because covalent binding is required |
Mus musculus |
2.3.1.37 |
K313H |
site-directed mutagenesis, mutants of erythroid-specific isoform, exchange of active site lysine residue 313, binding of pyridoxal 5'-phosphate and glycine noncovalently, reduced activity, because covalent binding is required |
Mus musculus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.37 |
Mus musculus |
- |
gene ALAS-E |
- |
2.3.1.37 |
Mus musculus |
- |
erythroid-specific isoform |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.3.1.37 |
recombinant wild-type and Lys313 mutants from Escherichia coli |
Mus musculus |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.3.1.37 |
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 |
covalent binding of pyridoxal 5'-phosphate and glycine to active site Lys131 is required for optimal activity |
Mus musculus |
|
2.3.1.37 |
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 |
Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation |
Mus musculus |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.1.37 |
succinyl-CoA + glycine |
- |
Mus musculus |
5-aminolevulinate + CoA + CO2 |
- |
? |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.3.1.37 |
8.5 |
- |
erythroid-specific isoform |
Mus musculus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.3.1.37 |
pyridoxal 5'-phosphate |
required |
Mus musculus |
|
2.3.1.37 |
pyridoxal 5'-phosphate |
content in ALAS-E wild-type and Lys313 mutants |
Mus musculus |
|