BRENDA - Enzyme Database show

Purification and structure of rat erythroid-specific delta-aminolevulinate synthase

Munakata, H.; Yamagami, T.; Nagai, T.; Yamamoto, M.; Hayashi, N.; J. Biochem. 114, 103-111 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
ATP
slight activation, erythroid-specific isoform
Rattus norvegicus
Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.37
erythroid-specific isoform ALAS-E, DNA-sequencing and sequence analysis, amino acid sequence determination of precursor protein
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
succinyl-CoA
non-specific isoform ALAS-N in absence of ATP
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.002
-
succinyl-CoA
erythroid-specific isoform
Rattus norvegicus
2.3.1.37
6.5
-
glycine
erythroid-specific isoform
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
additional information
-
amino acid sequence determination of the purified protein
Rattus norvegicus
2.3.1.37
49000
-
x * 49000, erythroid-specific isoform, SDS-PAGE
Rattus norvegicus
2.3.1.37
64840
-
erythroid-specific isoform precursor, DNA-sequence determination
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Rattus norvegicus
-
non-specific, housekeeping isoform ALAS-N and erythroid-specific isoform ALAS-E
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
erythroid-specific isoform from reticulocytes, papain digestion method
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
reticulocyte
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
1.87
-
purified erythroid-specific isoform
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486848
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486848
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
?
x * 49000, erythroid-specific isoform, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.37
30
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.6
-
erythroid-specific isoform
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
ATP
slight activation, erythroid-specific isoform
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
erythroid-specific isoform ALAS-E, DNA-sequencing and sequence analysis, amino acid sequence determination of precursor protein
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
succinyl-CoA
non-specific isoform ALAS-N in absence of ATP
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.002
-
succinyl-CoA
erythroid-specific isoform
Rattus norvegicus
2.3.1.37
6.5
-
glycine
erythroid-specific isoform
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
additional information
-
amino acid sequence determination of the purified protein
Rattus norvegicus
2.3.1.37
49000
-
x * 49000, erythroid-specific isoform, SDS-PAGE
Rattus norvegicus
2.3.1.37
64840
-
erythroid-specific isoform precursor, DNA-sequence determination
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
erythroid-specific isoform from reticulocytes, papain digestion method
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
reticulocyte
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
1.87
-
purified erythroid-specific isoform
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486848
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486848
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
?
x * 49000, erythroid-specific isoform, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.37
30
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.6
-
erythroid-specific isoform
Rattus norvegicus