BRENDA - Enzyme Database show

Hypoxic up-regulation of erythroid 5-aminolevulinate synthase

Hofer, T.; Wenger, R.H.; Kramer, M.F.; Ferreira, G.C.; Gassmann, M.; Blood 101, 348-350 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
additional information
iron depletion does not increase isoform II promotor activity
Mus musculus
2.3.1.37
O2
1%, 3fold increase in expression level, promotor activation in transiently transfected HeLa cells, hypoxia-inducible erythroid-isoform
Mus musculus
Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.37
transient expression of erythroid-specific isoform and promotor-mutant in HeLa cells, luciferase reporter gene
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.37
additional information
site-directed mutagenesis of hypoxia-inducible factor-1, i.e. HIF-1, binding site in promotor sequence -328/-318, reveales HIF-1 like activation of enzyme expression during hypoxia
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
succinyl-CoA + glycine
Mus musculus
rate-limiting enzyme of heme biosynthesis
5-aminolevulinate + CoA + CO2
-
Mus musculus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Mus musculus
-
erythroid-specific isoform; gene ALAS-2; hypoxia-inducible
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
erythroleukemia cell line
erythroid-specific isoform
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486846
Mus musculus
5-aminolevulinate + CoA + CO2
-
486846
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
rate-limiting enzyme of heme biosynthesis
486846
Mus musculus
5-aminolevulinate + CoA + CO2
-
486846
Mus musculus
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
additional information
iron depletion does not increase isoform II promotor activity
Mus musculus
2.3.1.37
O2
1%, 3fold increase in expression level, promotor activation in transiently transfected HeLa cells, hypoxia-inducible erythroid-isoform
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
transient expression of erythroid-specific isoform and promotor-mutant in HeLa cells, luciferase reporter gene
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.37
additional information
site-directed mutagenesis of hypoxia-inducible factor-1, i.e. HIF-1, binding site in promotor sequence -328/-318, reveales HIF-1 like activation of enzyme expression during hypoxia
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
succinyl-CoA + glycine
Mus musculus
rate-limiting enzyme of heme biosynthesis
5-aminolevulinate + CoA + CO2
-
Mus musculus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
erythroleukemia cell line
erythroid-specific isoform
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486846
Mus musculus
5-aminolevulinate + CoA + CO2
-
486846
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
rate-limiting enzyme of heme biosynthesis
486846
Mus musculus
5-aminolevulinate + CoA + CO2
-
486846
Mus musculus
?