BRENDA - Enzyme Database show

delta-Aminolevulinic acid synthetase of Rhodopseudomonas spheroides: purification and properties of the enzyme

Yubisui, T.; Yoneyama, Y.; Arch. Biochem. Biophys. 150, 77-85 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
EDTA
required
Rhodobacter sphaeroides
General Stability
EC Number
General Stability
Organism
2.3.1.37
freezing and thawing: precipitation and loss of activity
Rhodobacter sphaeroides
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
2-mercaptoethanol
-
Rhodobacter sphaeroides
2.3.1.37
Cu2+
at low concentration
Rhodobacter sphaeroides
2.3.1.37
Fe2+
-
Rhodobacter sphaeroides
2.3.1.37
Fe3+
slight
Rhodobacter sphaeroides
2.3.1.37
hemin
-
Rhodobacter sphaeroides
2.3.1.37
Hg2+
2-mercaptoethanol protects partly
Rhodobacter sphaeroides
2.3.1.37
iodoacetamide
-
Rhodobacter sphaeroides
2.3.1.37
Mg-protoporphyrin
-
Rhodobacter sphaeroides
2.3.1.37
N-ethylmaleimide
2-mercaptoethanol protect
Rhodobacter sphaeroides
2.3.1.37
p-chloromercuribenzoate
2-mercaptoethanol protect partly
Rhodobacter sphaeroides
2.3.1.37
protoporphyrin
weak
Rhodobacter sphaeroides
2.3.1.37
Zn2+
-
Rhodobacter sphaeroides
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
61000
-
gel filtration
Rhodobacter sphaeroides
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Rhodobacter sphaeroides
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
-
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.37
0.558
-
purified enzyme
Rhodobacter sphaeroides
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.37
5░C, 50 mM phosphate buffer, pH 7.2, 10 mM 2-mercaptoethanol, 20% loss of activity after 1 week
Rhodobacter sphaeroides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486842
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486842
Rhodobacter sphaeroides
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.2
-
-
Rhodobacter sphaeroides
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rhodobacter sphaeroides
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
EDTA
required
Rhodobacter sphaeroides
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rhodobacter sphaeroides
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.37
freezing and thawing: precipitation and loss of activity
Rhodobacter sphaeroides
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
2-mercaptoethanol
-
Rhodobacter sphaeroides
2.3.1.37
Cu2+
at low concentration
Rhodobacter sphaeroides
2.3.1.37
Fe2+
-
Rhodobacter sphaeroides
2.3.1.37
Fe3+
slight
Rhodobacter sphaeroides
2.3.1.37
hemin
-
Rhodobacter sphaeroides
2.3.1.37
Hg2+
2-mercaptoethanol protects partly
Rhodobacter sphaeroides
2.3.1.37
iodoacetamide
-
Rhodobacter sphaeroides
2.3.1.37
Mg-protoporphyrin
-
Rhodobacter sphaeroides
2.3.1.37
N-ethylmaleimide
2-mercaptoethanol protect
Rhodobacter sphaeroides
2.3.1.37
p-chloromercuribenzoate
2-mercaptoethanol protect partly
Rhodobacter sphaeroides
2.3.1.37
protoporphyrin
weak
Rhodobacter sphaeroides
2.3.1.37
Zn2+
-
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
61000
-
gel filtration
Rhodobacter sphaeroides
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
-
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.37
0.558
-
purified enzyme
Rhodobacter sphaeroides
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.37
5░C, 50 mM phosphate buffer, pH 7.2, 10 mM 2-mercaptoethanol, 20% loss of activity after 1 week
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486842
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486842
Rhodobacter sphaeroides
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.2
-
-
Rhodobacter sphaeroides