BRENDA - Enzyme Database show

Purification and some properties of delta-aminolevulinate acid synthases from Protaminobacter ruber and Rhodopseudomonas speroides

Sato, K.; Ishida, K.; Mutsushika, O.; Shimizu, S.; Agric. Biol. Chem. 49, 3415-3421 (1985)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
vitamin B12
stimulates
Rhodobacter sphaeroides
2.3.1.37
vitamin B12
stimulates
Serratia plymuthica
General Stability
EC Number
General Stability
Organism
2.3.1.37
holoenzyme is more stable than apoenzyme
Rhodobacter sphaeroides
2.3.1.37
holoenzyme is more stable than apoenzyme
Serratia plymuthica
2.3.1.37
pyridoxal 5'-phosphate stabilizes
Rhodobacter sphaeroides
2.3.1.37
pyridoxal 5'-phosphate stabilizes
Serratia plymuthica
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
hemin
no inhibition
Serratia plymuthica
2.3.1.37
hemin
-
Rhodobacter sphaeroides
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.00077
-
pyridoxal 5'-phosphate
-
Serratia plymuthica
2.3.1.37
0.015
-
pyridoxal 5'-phosphate
-
Rhodobacter sphaeroides
2.3.1.37
1.05
-
glycine
-
Serratia plymuthica
2.3.1.37
8.33
-
glycine
-
Rhodobacter sphaeroides
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
87000
-
gel filtration
Rhodobacter sphaeroides
2.3.1.37
97000
-
gel filtration
Serratia plymuthica
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Rhodobacter sphaeroides
-
-
-
2.3.1.37
Serratia plymuthica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
-
Serratia plymuthica
2.3.1.37
-
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.35
-
purified enzyme
Serratia plymuthica
2.3.1.37
1.34
-
purified enzyme
Rhodobacter sphaeroides
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.37
4°C, 30 min, 50% loss of apoenzyme of Protaminobacter ruber
Serratia plymuthica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486839
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486839
Rhodobacter sphaeroides
?
2.3.1.37
succinyl-CoA + glycine
-
486839
Serratia plymuthica
5-aminolevulinate + CoA + CO2
-
486839
Serratia plymuthica
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.37
37
-
assay at
Rhodobacter sphaeroides
2.3.1.37
37
-
assay at
Serratia plymuthica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
8
-
-
Rhodobacter sphaeroides
2.3.1.37
8
-
-
Serratia plymuthica
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Serratia plymuthica
2.3.1.37
pyridoxal 5'-phosphate
required
Rhodobacter sphaeroides
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
vitamin B12
stimulates
Rhodobacter sphaeroides
2.3.1.37
vitamin B12
stimulates
Serratia plymuthica
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Serratia plymuthica
2.3.1.37
pyridoxal 5'-phosphate
required
Rhodobacter sphaeroides
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.37
holoenzyme is more stable than apoenzyme
Rhodobacter sphaeroides
2.3.1.37
holoenzyme is more stable than apoenzyme
Serratia plymuthica
2.3.1.37
pyridoxal 5'-phosphate stabilizes
Rhodobacter sphaeroides
2.3.1.37
pyridoxal 5'-phosphate stabilizes
Serratia plymuthica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
hemin
no inhibition
Serratia plymuthica
2.3.1.37
hemin
-
Rhodobacter sphaeroides
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.00077
-
pyridoxal 5'-phosphate
-
Serratia plymuthica
2.3.1.37
0.015
-
pyridoxal 5'-phosphate
-
Rhodobacter sphaeroides
2.3.1.37
1.05
-
glycine
-
Serratia plymuthica
2.3.1.37
8.33
-
glycine
-
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
87000
-
gel filtration
Rhodobacter sphaeroides
2.3.1.37
97000
-
gel filtration
Serratia plymuthica
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
-
Serratia plymuthica
2.3.1.37
-
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.35
-
purified enzyme
Serratia plymuthica
2.3.1.37
1.34
-
purified enzyme
Rhodobacter sphaeroides
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.37
4°C, 30 min, 50% loss of apoenzyme of Protaminobacter ruber
Serratia plymuthica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486839
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486839
Rhodobacter sphaeroides
?
2.3.1.37
succinyl-CoA + glycine
-
486839
Serratia plymuthica
5-aminolevulinate + CoA + CO2
-
486839
Serratia plymuthica
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.37
37
-
assay at
Rhodobacter sphaeroides
2.3.1.37
37
-
assay at
Serratia plymuthica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
8
-
-
Rhodobacter sphaeroides
2.3.1.37
8
-
-
Serratia plymuthica