BRENDA - Enzyme Database show

Purification and some properties of two forms of delta-aminolevulinate synthase from rat liver cytosol

Ohashi, A.; Kikuchi, G.; J. Biochem. 85, 239-247 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
Mg2+
inhibition of complex form, stimulation of stripped form
Rattus norvegicus
2.3.1.37
Na+
inhibition of complex form, stimulation of stripped form
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.02
-
succinyl-CoA
-
Rattus norvegicus
2.3.1.37
12
-
glycine
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
Mg2+
inhibition of complex form, stimulation of stripped form
Rattus norvegicus
2.3.1.37
Na+
inhibition of complex form, stimulation of stripped form
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
51000
-
2 * 51000, cytosol, SDS-PAGE
Rattus norvegicus
2.3.1.37
107000
110000
stripped form, sucrose density gradient centrifugation, gel filtration, disc gel electrophoresis
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
2 forms: a complex form and a stripped form that represents a catalytically active protein component of the complex form
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.091
-
purified enzyme
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486837
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486837
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 51000, cytosol, SDS-PAGE
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
Mg2+
inhibition of complex form, stimulation of stripped form
Rattus norvegicus
2.3.1.37
Na+
inhibition of complex form, stimulation of stripped form
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.02
-
succinyl-CoA
-
Rattus norvegicus
2.3.1.37
12
-
glycine
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
Mg2+
inhibition of complex form, stimulation of stripped form
Rattus norvegicus
2.3.1.37
Na+
inhibition of complex form, stimulation of stripped form
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
51000
-
2 * 51000, cytosol, SDS-PAGE
Rattus norvegicus
2.3.1.37
107000
110000
stripped form, sucrose density gradient centrifugation, gel filtration, disc gel electrophoresis
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
2 forms: a complex form and a stripped form that represents a catalytically active protein component of the complex form
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.091
-
purified enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486837
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486837
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 51000, cytosol, SDS-PAGE
Rattus norvegicus