BRENDA - Enzyme Database show

Isolation and properties of 5-aminolevulinate synthase from the yeast Saccharomyces cerevisiae

Volland, C.; Felix, F.; Eur. J. Biochem. 142, 551-557 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
ferroheme
not heme
Saccharomyces cerevisiae
2.3.1.37
hemin
-
Saccharomyces cerevisiae
2.3.1.37
N-ethylmaleimide
-
Saccharomyces cerevisiae
2.3.1.37
p-chloromercuribenzoate
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.002
-
succinyl-CoA
-
Saccharomyces cerevisiae
2.3.1.37
3
-
glycine
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
mitochondrion
-
Saccharomyces cerevisiae
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
53000
-
2 * 53000, SDS-PAGE
Saccharomyces cerevisiae
2.3.1.37
93000
-
gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.65
-
purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486835
Saccharomyces cerevisiae
5-aminolevulinate + CoA + CO2
-
486835
Saccharomyces cerevisiae
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 53000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.37
30
-
assay at
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.4
-
-
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.37
5.8
8.6
about 50% of activity maximum at pH 5.8 and pH 8.6
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
ferroheme
not heme
Saccharomyces cerevisiae
2.3.1.37
hemin
-
Saccharomyces cerevisiae
2.3.1.37
N-ethylmaleimide
-
Saccharomyces cerevisiae
2.3.1.37
p-chloromercuribenzoate
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.002
-
succinyl-CoA
-
Saccharomyces cerevisiae
2.3.1.37
3
-
glycine
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
mitochondrion
-
Saccharomyces cerevisiae
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
53000
-
2 * 53000, SDS-PAGE
Saccharomyces cerevisiae
2.3.1.37
93000
-
gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.65
-
purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486835
Saccharomyces cerevisiae
5-aminolevulinate + CoA + CO2
-
486835
Saccharomyces cerevisiae
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 53000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.37
30
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.4
-
-
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.37
5.8
8.6
about 50% of activity maximum at pH 5.8 and pH 8.6
Saccharomyces cerevisiae