BRENDA - Enzyme Database show

Purification and some properties of delta-aminolevulinate synthase from the rat liver cytosol fraction and immunochemical identity of the cytosolic enzyme and the mitochondrial enzyme

Nakakuki, M.; Yamauchi, K.; Hayashi, N.; Kikuchi, G.; J. Biol. Chem. 255, 1738-1745 (1980)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.011
-
succinyl-CoA
-
Rattus norvegicus
2.3.1.37
2.5
-
glycine
papain untreated enzyme
Rattus norvegicus
2.3.1.37
7.5
-
glycine
papain treated enzyme
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
51000
-
2 * 51000, cytosol, SDS-PAGE
Rattus norvegicus
2.3.1.37
110000
-
sucrose density gradient centrifugation, gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.37
1.22
-
purified enzyme
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486834
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486834
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 51000, cytosol, SDS-PAGE
Rattus norvegicus
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.3.1.37
30
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.6
-
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.011
-
succinyl-CoA
-
Rattus norvegicus
2.3.1.37
2.5
-
glycine
papain untreated enzyme
Rattus norvegicus
2.3.1.37
7.5
-
glycine
papain treated enzyme
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
51000
-
2 * 51000, cytosol, SDS-PAGE
Rattus norvegicus
2.3.1.37
110000
-
sucrose density gradient centrifugation, gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.37
1.22
-
purified enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486834
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486834
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 51000, cytosol, SDS-PAGE
Rattus norvegicus
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.3.1.37
30
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.6
-
-
Rattus norvegicus