BRENDA - Enzyme Database show

delta-Aminolevulinic acid synthetase from rat liver mitochondria. Purification and properties

Paterniti, J.R.; Beattie, D.S.; J. Biol. Chem. 254, 6112-6118 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
hemin
feed-back inhibition
Rattus norvegicus
2.3.1.37
monovalent cations
in Mg2+-containing medium: Na+ or Li+ at 100 mM have no effect on activity, higher concentrations are totally inhibitory
Rattus norvegicus
2.3.1.37
additional information
presence of high salt concentration in the sample buffer and running buffer of aggregated enzyme preparation results in inactivation
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
5
-
succinyl-CoA
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
Li+
in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory
Rattus norvegicus
2.3.1.37
Na+
in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
58000
-
2 * 58000, mitochondria, SDS-PAGE
Rattus norvegicus
2.3.1.37
120000
-
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.035
-
purified enzyme
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.37
-20°C, 20% loss of activity after 1 month
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486833
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486833
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 58000, mitochondria, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.5
-
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
hemin
feed-back inhibition
Rattus norvegicus
2.3.1.37
monovalent cations
in Mg2+-containing medium: Na+ or Li+ at 100 mM have no effect on activity, higher concentrations are totally inhibitory
Rattus norvegicus
2.3.1.37
additional information
presence of high salt concentration in the sample buffer and running buffer of aggregated enzyme preparation results in inactivation
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
5
-
succinyl-CoA
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
Li+
in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory
Rattus norvegicus
2.3.1.37
Na+
in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
58000
-
2 * 58000, mitochondria, SDS-PAGE
Rattus norvegicus
2.3.1.37
120000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.035
-
purified enzyme
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.37
-20°C, 20% loss of activity after 1 month
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486833
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486833
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 58000, mitochondria, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.5
-
-
Rattus norvegicus