BRENDA - Enzyme Database show

delta-Aminolevulinic acid synthase from chick embryo liver mitochondria. I. Purification and some properties

Whiting, M.J.; Granick, S.; J. Biol. Chem. 251, 1340-1346 (1976)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
divalent cations
Mg2+ or Ca2+: optimal at concentration of 50 mM, inhibition above 200 mM
Gallus gallus
2.3.1.37
hemin
-
Gallus gallus
2.3.1.37
monovalent cations
Na+ or Li+: optimal at 0.5 M, not inhibitory up to 1 M
Gallus gallus
2.3.1.37
p-chloromercuribenzoate
-
Gallus gallus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
Ca2+
optimal at concentration of 50 mM, inhibition above 200 mM
Gallus gallus
2.3.1.37
Li+
in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory; optimal at 0.5 M, not inhibitory up to 1 M
Gallus gallus
2.3.1.37
Mg2+
optimal at concentration of 50 mM, inhibition above 200 mM
Gallus gallus
2.3.1.37
Na+
in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory; optimal at 0.5 M, not inhibitory up to 1 M
Gallus gallus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
49000
-
2 * 49000, SDS-PAGE
Gallus gallus
2.3.1.37
87000
-
gel filtration
Gallus gallus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Gallus gallus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
-
Gallus gallus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
embryo
-
Gallus gallus
-
2.3.1.37
liver
fetal
Gallus gallus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
additional information
-
-
Gallus gallus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.37
-15°C, 20 mM potassium phosphate buffer, pH 7.6, 1 mM dithioerythritol, 0.01 mM pyridoxal 5'-phosphate, 30% loss of activity after 2 weeks
Gallus gallus
2.3.1.37
4°C, 20 mM potassium phosphate buffer, pH 7.6, 1 mM dithioerythritol, 0.01 mM pyridoxal 5'-phosphate, 20% loss of activity after 2 weeks
Gallus gallus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486832
Gallus gallus
5-aminolevulinate + CoA + CO2
-
486832
Gallus gallus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 49000, SDS-PAGE
Gallus gallus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Gallus gallus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.37
0.035
-
hemin
-
Gallus gallus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
required
Gallus gallus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
divalent cations
Mg2+ or Ca2+: optimal at concentration of 50 mM, inhibition above 200 mM
Gallus gallus
2.3.1.37
hemin
-
Gallus gallus
2.3.1.37
monovalent cations
Na+ or Li+: optimal at 0.5 M, not inhibitory up to 1 M
Gallus gallus
2.3.1.37
p-chloromercuribenzoate
-
Gallus gallus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.37
0.035
-
hemin
-
Gallus gallus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
Ca2+
optimal at concentration of 50 mM, inhibition above 200 mM
Gallus gallus
2.3.1.37
Li+
in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory; optimal at 0.5 M, not inhibitory up to 1 M
Gallus gallus
2.3.1.37
Mg2+
optimal at concentration of 50 mM, inhibition above 200 mM
Gallus gallus
2.3.1.37
Na+
in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory; optimal at 0.5 M, not inhibitory up to 1 M
Gallus gallus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
49000
-
2 * 49000, SDS-PAGE
Gallus gallus
2.3.1.37
87000
-
gel filtration
Gallus gallus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
-
Gallus gallus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
embryo
-
Gallus gallus
-
2.3.1.37
liver
fetal
Gallus gallus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
additional information
-
-
Gallus gallus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.37
-15°C, 20 mM potassium phosphate buffer, pH 7.6, 1 mM dithioerythritol, 0.01 mM pyridoxal 5'-phosphate, 30% loss of activity after 2 weeks
Gallus gallus
2.3.1.37
4°C, 20 mM potassium phosphate buffer, pH 7.6, 1 mM dithioerythritol, 0.01 mM pyridoxal 5'-phosphate, 20% loss of activity after 2 weeks
Gallus gallus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486832
Gallus gallus
5-aminolevulinate + CoA + CO2
-
486832
Gallus gallus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 49000, SDS-PAGE
Gallus gallus