BRENDA - Enzyme Database show

Expression of mammalian 5-aminolevulinate synthase in Escherichia coli. Overproduction, purification, and characterization

Ferreira, G.C.; Dailey, H.A.; J. Biol. Chem. 268, 584-590 (1993)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.37
overexpression of mouse erythroid enzyme in Escherichia coli
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.055
-
succinyl-CoA
recombinant protein
Mus musculus
2.3.1.37
51
-
glycine
recombinant protein
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
56000
-
2 * 56000, mouse enzyme expressed in Escherichia coli, SDS-PAGE
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
succinyl-CoA + glycine
Mus musculus
first step in heme biosynthesis pathway
5-aminolevulinate + CoA + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Mus musculus
-
erythroid-specific isoform; gene ALAS-2
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
recombinant from E. coli
Mus musculus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.37
additional information
-
-
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486824
Mus musculus
5-aminolevulinate + CoA + CO2
-
486824
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
first step in heme biosynthesis pathway
486824
Mus musculus
5-aminolevulinate + CoA + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 56000, mouse enzyme expressed in Escherichia coli, SDS-PAGE
Mus musculus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.0095
-
succinyl-CoA
-
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
overexpression of mouse erythroid enzyme in Escherichia coli
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.055
-
succinyl-CoA
recombinant protein
Mus musculus
2.3.1.37
51
-
glycine
recombinant protein
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
56000
-
2 * 56000, mouse enzyme expressed in Escherichia coli, SDS-PAGE
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
succinyl-CoA + glycine
Mus musculus
first step in heme biosynthesis pathway
5-aminolevulinate + CoA + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
recombinant from E. coli
Mus musculus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.37
additional information
-
-
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486824
Mus musculus
5-aminolevulinate + CoA + CO2
-
486824
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
first step in heme biosynthesis pathway
486824
Mus musculus
5-aminolevulinate + CoA + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 56000, mouse enzyme expressed in Escherichia coli, SDS-PAGE
Mus musculus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.0095
-
succinyl-CoA
-
Mus musculus