BRENDA - Enzyme Database show

Purification of a specific activator of solubilized delta-aminolevulinic acid synthetase from rat liver mitochondria

Simpson, D.M.; Beattie, D.S.; J. Biol. Chem. 255, 1630-1634 (1980)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
Activator protein
from rat liver mitochondria possibly activates by formation of high molecular weight aggregates of delta-aminolevulinic acid synthetase
Rattus norvegicus
2.3.1.37
native activator protein
purified from hepatic extract
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
54000
-
2 * 54000, SDS-PAGE
Rattus norvegicus
2.3.1.37
58000
-
monomer, gel filtration
Rattus norvegicus
2.3.1.37
118000
-
dimer, gel filtration
Rattus norvegicus
2.3.1.37
218000
-
tetramer of enzyme and native activator protein, gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486822
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486822
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 54000, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
additional information
-
pI: 7.5
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
Activator protein
from rat liver mitochondria possibly activates by formation of high molecular weight aggregates of delta-aminolevulinic acid synthetase
Rattus norvegicus
2.3.1.37
native activator protein
purified from hepatic extract
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
54000
-
2 * 54000, SDS-PAGE
Rattus norvegicus
2.3.1.37
58000
-
monomer, gel filtration
Rattus norvegicus
2.3.1.37
118000
-
dimer, gel filtration
Rattus norvegicus
2.3.1.37
218000
-
tetramer of enzyme and native activator protein, gel filtration
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486822
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486822
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 54000, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
additional information
-
pI: 7.5
Rattus norvegicus