BRENDA - Enzyme Database show

Purification of 5-aminolevulinate synthase

Jordan, P.M.; Laghai-Newton, A.; Methods Enzymol. 123, 435-443 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
aminomalonate
-
Rhodobacter sphaeroides
2.3.1.37
Cu2+
-
Rhodobacter sphaeroides
2.3.1.37
Fe2+
-
Rhodobacter sphaeroides
2.3.1.37
hemin
reversible, noncompetitive
Rhodobacter sphaeroides
2.3.1.37
p-chloromercuribenzoate
-
Rhodobacter sphaeroides
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
49000
-
2 * 49000, SDS-PAGE
Rhodobacter sphaeroides
2.3.1.37
81000
-
gel filtration
Rhodobacter sphaeroides
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Rhodobacter sphaeroides
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
-
Rhodobacter sphaeroides
Reaction
EC Number
Reaction
Commentary
Organism
2.3.1.37
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2
mechanism
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
2.22
-
purified enzyme
Rhodobacter sphaeroides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
acetyl-CoA + glycine
low activity
486819
Rhodobacter sphaeroides
?
-
-
-
?
2.3.1.37
butyryl-CoA + glycine
low activity
486819
Rhodobacter sphaeroides
?
-
-
-
?
2.3.1.37
propionyl-CoA + glycine
low activity
486819
Rhodobacter sphaeroides
?
-
-
-
?
2.3.1.37
succinyl-CoA + glycine
absolute requirement for glycine
486819
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486819
Rhodobacter sphaeroides
?
2.3.1.37
succinyl-CoA + glycine
enzyme utilizes acetyl-CoA, propionyl-CoA and butyryl-CoA at much lower rates
486819
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486819
Rhodobacter sphaeroides
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 49000, SDS-PAGE
Rhodobacter sphaeroides
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.37
37
-
assay at
Rhodobacter sphaeroides
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
fully active with 0.05 mM pyridoxal 5'-phosphate; weekly bound
Rhodobacter sphaeroides
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
fully active with 0.05 mM pyridoxal 5'-phosphate; weekly bound
Rhodobacter sphaeroides
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
aminomalonate
-
Rhodobacter sphaeroides
2.3.1.37
Cu2+
-
Rhodobacter sphaeroides
2.3.1.37
Fe2+
-
Rhodobacter sphaeroides
2.3.1.37
hemin
reversible, noncompetitive
Rhodobacter sphaeroides
2.3.1.37
p-chloromercuribenzoate
-
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
49000
-
2 * 49000, SDS-PAGE
Rhodobacter sphaeroides
2.3.1.37
81000
-
gel filtration
Rhodobacter sphaeroides
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
-
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
2.22
-
purified enzyme
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
acetyl-CoA + glycine
low activity
486819
Rhodobacter sphaeroides
?
-
-
-
?
2.3.1.37
butyryl-CoA + glycine
low activity
486819
Rhodobacter sphaeroides
?
-
-
-
?
2.3.1.37
propionyl-CoA + glycine
low activity
486819
Rhodobacter sphaeroides
?
-
-
-
?
2.3.1.37
succinyl-CoA + glycine
absolute requirement for glycine
486819
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486819
Rhodobacter sphaeroides
?
2.3.1.37
succinyl-CoA + glycine
enzyme utilizes acetyl-CoA, propionyl-CoA and butyryl-CoA at much lower rates
486819
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486819
Rhodobacter sphaeroides
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
2 * 49000, SDS-PAGE
Rhodobacter sphaeroides
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.37
37
-
assay at
Rhodobacter sphaeroides