BRENDA - Enzyme Database show

Purification of hepatic mitochondrial 5-aminolevulinate synthase

Borthwick, I.A.; Srivastava, G.; Pirola, B.A.; May, B.K.; Elliott, W.H.; Methods Enzymol. 123, 395-401 (1986)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
mitochondrial inner membrane
loosely bound
Gallus gallus
5743
-
2.3.1.37
mitochondrial inner membrane
loosely bound
Rattus norvegicus
5743
-
2.3.1.37
mitochondrial matrix
-
Gallus gallus
5759
-
2.3.1.37
mitochondrial matrix
-
Rattus norvegicus
5759
-
2.3.1.37
mitochondrion
-
Gallus gallus
5739
-
2.3.1.37
mitochondrion
-
Rattus norvegicus
5739
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
succinyl-CoA + glycine
Gallus gallus
rate-limiting enzyme of heme biosynthesis
5-aminolevulinate + CoA + CO2
-
-
?
2.3.1.37
succinyl-CoA + glycine
Rattus norvegicus
rate-limiting enzyme of heme biosynthesis
5-aminolevulinate + CoA + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Gallus gallus
-
-
-
2.3.1.37
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
-
Gallus gallus
2.3.1.37
difficulties due to the tendency of the enzyme to form large MW aggregates and to its susceptibility to proteolytic degradation
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
embryo
-
Gallus gallus
-
2.3.1.37
embryo
-
Rattus norvegicus
-
2.3.1.37
liver
fetal
Gallus gallus
-
2.3.1.37
liver
fetal
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.37
0.16
-
purified enzyme
Rattus norvegicus
2.3.1.37
0.58
-
purified enzyme
Gallus gallus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.37
-195C, following an initial loss of 20% of activity due to freeze-thawing the enzyme loses no further activity for at least 6 months
Gallus gallus
2.3.1.37
-195C, following an initial loss of 20% of activity due to freeze-thawing the enzyme loses no further activity for at least 6 months
Rattus norvegicus
2.3.1.37
4C, 50 mM Tris-HCl, 100 mM glycine, 0.1 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, pH 7.6, 5 mM 5'-AMP, stable for at least 4 months
Gallus gallus
2.3.1.37
4C, 50 mM Tris-HCl, 100 mM glycine, 0.1 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, pH 7.6, 5 mM 5'-AMP, stable for at least 4 months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486818
Gallus gallus
5-aminolevulinate + CoA + CO2
-
486818
Gallus gallus
?
2.3.1.37
succinyl-CoA + glycine
-
486818
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486818
Rattus norvegicus
?
2.3.1.37
succinyl-CoA + glycine
rate-limiting enzyme of heme biosynthesis
486818
Gallus gallus
5-aminolevulinate + CoA + CO2
-
-
-
?
2.3.1.37
succinyl-CoA + glycine
rate-limiting enzyme of heme biosynthesis
486818
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.37
37
-
assay at
Gallus gallus
2.3.1.37
37
-
assay at
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Gallus gallus
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Gallus gallus
2.3.1.37
pyridoxal 5'-phosphate
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.37
mitochondrial inner membrane
loosely bound
Gallus gallus
5743
-
2.3.1.37
mitochondrial inner membrane
loosely bound
Rattus norvegicus
5743
-
2.3.1.37
mitochondrial matrix
-
Gallus gallus
5759
-
2.3.1.37
mitochondrial matrix
-
Rattus norvegicus
5759
-
2.3.1.37
mitochondrion
-
Gallus gallus
5739
-
2.3.1.37
mitochondrion
-
Rattus norvegicus
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
succinyl-CoA + glycine
Gallus gallus
rate-limiting enzyme of heme biosynthesis
5-aminolevulinate + CoA + CO2
-
-
?
2.3.1.37
succinyl-CoA + glycine
Rattus norvegicus
rate-limiting enzyme of heme biosynthesis
5-aminolevulinate + CoA + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
-
Gallus gallus
2.3.1.37
difficulties due to the tendency of the enzyme to form large MW aggregates and to its susceptibility to proteolytic degradation
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
embryo
-
Gallus gallus
-
2.3.1.37
embryo
-
Rattus norvegicus
-
2.3.1.37
liver
fetal
Gallus gallus
-
2.3.1.37
liver
fetal
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.37
0.16
-
purified enzyme
Rattus norvegicus
2.3.1.37
0.58
-
purified enzyme
Gallus gallus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.37
-195C, following an initial loss of 20% of activity due to freeze-thawing the enzyme loses no further activity for at least 6 months
Gallus gallus
2.3.1.37
-195C, following an initial loss of 20% of activity due to freeze-thawing the enzyme loses no further activity for at least 6 months
Rattus norvegicus
2.3.1.37
4C, 50 mM Tris-HCl, 100 mM glycine, 0.1 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, pH 7.6, 5 mM 5'-AMP, stable for at least 4 months
Gallus gallus
2.3.1.37
4C, 50 mM Tris-HCl, 100 mM glycine, 0.1 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, pH 7.6, 5 mM 5'-AMP, stable for at least 4 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486818
Gallus gallus
5-aminolevulinate + CoA + CO2
-
486818
Gallus gallus
?
2.3.1.37
succinyl-CoA + glycine
-
486818
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
486818
Rattus norvegicus
?
2.3.1.37
succinyl-CoA + glycine
rate-limiting enzyme of heme biosynthesis
486818
Gallus gallus
5-aminolevulinate + CoA + CO2
-
-
-
?
2.3.1.37
succinyl-CoA + glycine
rate-limiting enzyme of heme biosynthesis
486818
Rattus norvegicus
5-aminolevulinate + CoA + CO2
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.37
37
-
assay at
Gallus gallus
2.3.1.37
37
-
assay at
Rattus norvegicus