BRENDA - Enzyme Database show

Aminolaevulinate synthetase of Micrococcus denitrificans. Purification and properties of the enzyme, and the effect of growth conditions on the enzyme activity in cells

Tait, G.H.; Biochem. J. 131, 389-403 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
Chloramphenicol
-
Paracoccus denitrificans
2.3.1.37
hemin
feed-back inhibition
Paracoccus denitrificans
2.3.1.37
protoporphyrin
-
Paracoccus denitrificans
2.3.1.37
puromycin
-
Paracoccus denitrificans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.01
-
succinyl-CoA
-
Paracoccus denitrificans
2.3.1.37
10
-
succinyl-CoA
-
Paracoccus denitrificans
2.3.1.37
12
-
glycine
-
Paracoccus denitrificans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
68000
-
gel filtration
Paracoccus denitrificans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Paracoccus denitrificans
-
in cells grown anaerobically and in iron-containing nitrate medium, overview
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
high activity in cells grown anaerobically in defined medium, low in cells grown in an iron-deficient medium and in cells grown aerobically
Paracoccus denitrificans
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
cell culture
high activity in cells grown anaerobically in defined medium, low in cells grown in an iron-deficient medium and in cells grown aerobically
Paracoccus denitrificans
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.0037
-
crude enzyme extract
Paracoccus denitrificans
2.3.1.37
0.62
-
purified enzyme
Paracoccus denitrificans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486810
Paracoccus denitrificans
5-aminolevulinate + CoA + CO2
-
486810
Paracoccus denitrificans
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7
-
0.1 M phosphate buffer
Paracoccus denitrificans
2.3.1.37
7.5
-
0.1 M Tris-HCl buffer
Paracoccus denitrificans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Paracoccus denitrificans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Paracoccus denitrificans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
Chloramphenicol
-
Paracoccus denitrificans
2.3.1.37
hemin
feed-back inhibition
Paracoccus denitrificans
2.3.1.37
protoporphyrin
-
Paracoccus denitrificans
2.3.1.37
puromycin
-
Paracoccus denitrificans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
0.01
-
succinyl-CoA
-
Paracoccus denitrificans
2.3.1.37
10
-
succinyl-CoA
-
Paracoccus denitrificans
2.3.1.37
12
-
glycine
-
Paracoccus denitrificans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
68000
-
gel filtration
Paracoccus denitrificans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
high activity in cells grown anaerobically in defined medium, low in cells grown in an iron-deficient medium and in cells grown aerobically
Paracoccus denitrificans
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
cell culture
high activity in cells grown anaerobically in defined medium, low in cells grown in an iron-deficient medium and in cells grown aerobically
Paracoccus denitrificans
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.0037
-
crude enzyme extract
Paracoccus denitrificans
2.3.1.37
0.62
-
purified enzyme
Paracoccus denitrificans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486810
Paracoccus denitrificans
5-aminolevulinate + CoA + CO2
-
486810
Paracoccus denitrificans
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7
-
0.1 M phosphate buffer
Paracoccus denitrificans
2.3.1.37
7.5
-
0.1 M Tris-HCl buffer
Paracoccus denitrificans