BRENDA - Enzyme Database show

delta-Aminolevulinic acid synthetase from cold-stored potatoes

Ramaswamy, N.K.; Madhusudanan Nair, P.; Biochim. Biophys. Acta 293, 269-277 (1973)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
ATP
activates in assays with crude enzyme extracts
Solanum tuberosum
2.3.1.37
CoA
activates
Solanum tuberosum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
chlorophyllide a
-
Solanum tuberosum
2.3.1.37
hemin
-
Solanum tuberosum
2.3.1.37
light
above 30 lux intensity
Solanum tuberosum
2.3.1.37
additional information
pheophytin and chlorophyll are not inhibitory
Solanum tuberosum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
Mg2+
activates
Solanum tuberosum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
succinyl-CoA + glycine
Solanum tuberosum
-
5-aminolevulinate + CoA + CO2
common intermediate in the biosynthesis of chlorophyll and heme
Solanum tuberosum
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Solanum tuberosum
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
tuber
dim light and cold-stored, green skin peeling
Solanum tuberosum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
additional information
-
EDTA required for the assay to inhibit 5-aminolevulinate dehydratase
Solanum tuberosum
2.3.1.37
0.0057
-
maximal enzyme activity after 3 weeks of storage of tubers at 0-15°C at 30 lux
Solanum tuberosum
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.37
-30°C, complete loss of activity overnight
Solanum tuberosum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486809
Solanum tuberosum
5-aminolevulinate + CoA + CO2
-
486809
Solanum tuberosum
?
2.3.1.37
succinyl-CoA + glycine
-
486809
Solanum tuberosum
5-aminolevulinate + CoA + CO2
common intermediate in the biosynthesis of chlorophyll and heme
486809
Solanum tuberosum
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.37
15
-
-
Solanum tuberosum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.37
additional information
-
room temperature, inactivation within 30 min
Solanum tuberosum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7
-
-
Solanum tuberosum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
activates; required
Solanum tuberosum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
ATP
activates in assays with crude enzyme extracts
Solanum tuberosum
2.3.1.37
CoA
activates
Solanum tuberosum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
activates; required
Solanum tuberosum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
chlorophyllide a
-
Solanum tuberosum
2.3.1.37
hemin
-
Solanum tuberosum
2.3.1.37
light
above 30 lux intensity
Solanum tuberosum
2.3.1.37
additional information
pheophytin and chlorophyll are not inhibitory
Solanum tuberosum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
Mg2+
activates
Solanum tuberosum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
succinyl-CoA + glycine
Solanum tuberosum
-
5-aminolevulinate + CoA + CO2
common intermediate in the biosynthesis of chlorophyll and heme
Solanum tuberosum
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.37
tuber
dim light and cold-stored, green skin peeling
Solanum tuberosum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
additional information
-
EDTA required for the assay to inhibit 5-aminolevulinate dehydratase
Solanum tuberosum
2.3.1.37
0.0057
-
maximal enzyme activity after 3 weeks of storage of tubers at 0-15°C at 30 lux
Solanum tuberosum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.37
-30°C, complete loss of activity overnight
Solanum tuberosum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486809
Solanum tuberosum
5-aminolevulinate + CoA + CO2
-
486809
Solanum tuberosum
?
2.3.1.37
succinyl-CoA + glycine
-
486809
Solanum tuberosum
5-aminolevulinate + CoA + CO2
common intermediate in the biosynthesis of chlorophyll and heme
486809
Solanum tuberosum
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.37
15
-
-
Solanum tuberosum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.37
additional information
-
room temperature, inactivation within 30 min
Solanum tuberosum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7
-
-
Solanum tuberosum