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Literature summary extracted from

  • Sakanyan, V.; Charlier, D.; Legrain, C.; Kochikyan, A.; Mett, I.; Pierard, A.; Glansdorff, N.
    Primary structure, partial purification and regulation of key enzymes of the acetyl cycle of arginine biosynthesis in Bacillus stearothermophilus: dual function of ornithine acetyltransferase (1993), J. Gen. Microbiol., 139, 393-402.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.35 expression in Escherichia coli Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.35 L-ornithine
-
 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.35 0.4
-
 alpha-N-acetyl-L-ornithine
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.35 110000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Geobacillus stearothermophilus first step in arginine biosynthesis L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Geobacillus stearothermophilus bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis L-ornithine + N-acetyl-L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.35 Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + H2O
-
 Geobacillus stearothermophilus L-ornithine + acetate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate
-
 Geobacillus stearothermophilus L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate first step in arginine biosynthesis Geobacillus stearothermophilus L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis Geobacillus stearothermophilus L-ornithine + N-acetyl-L-glutamate
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.35 20 65
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.35 70
-
 inactivation after 20 min Geobacillus stearothermophilus