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Literature summary extracted from

  • Kershaw, N.J.; McNaughton, H.J.; Hewitson, K.S.; Hernandez, H.; Griffin, J.; Hughes, C.; Greaves, P.; Barton, B.; Robinson, C.V.; Schofield, C.J.
    ORF6 from the clavulanic acid gene cluster of Streptomyces clavuligerus has ornithine acetyltransferase activity (2002), Eur. J. Biochem., 269, 2052-2059.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.35 overexpression in Escherichia coli Streptomyces clavuligerus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.35 3.6
-
 alpha-N-acetyl-L-ornithine
-
 Streptomyces clavuligerus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.35 19000
-
 alpha2beta2, 2 * 19000 + 2 * 25000, SDS-PAGE Streptomyces clavuligerus
2.3.1.35 25000
-
 alpha2beta2, 2 * 19000 + 2 * 25000, SDS-PAGE Streptomyces clavuligerus
2.3.1.35 83000
-
 gel filtration Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Streptomyces clavuligerus first step in arginine biosynthesis L-ornithine + N-acetyl-L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.35 Streptomyces clavuligerus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.35
-
 Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.35 0.087
-
-
 Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate specific for L-glutamate Streptomyces clavuligerus L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate first step in arginine biosynthesis Streptomyces clavuligerus L-ornithine + N-acetyl-L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.35 heterotetramer alpha2beta2, 2 * 19000 + 2 * 25000, SDS-PAGE Streptomyces clavuligerus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.35 7.5 8
-
 Streptomyces clavuligerus