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Literature summary extracted from

  • Paik, W.K.; Kim, S.
    Enzyme synthesis of epsilon-N-acetyl-L-lysine (1964), Arch. Biochem. Biophys., 108, 221-229.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.32 additional information no inhibition by cysteine, glutathione, ascorbic acid, Zn2+, Mn2+, Co2+, Ni2+, Fe3+ or acetate Bos taurus
2.3.1.32 phosphate above 0.1 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.32 1.32
-
 L-ornithine
-
 Bos taurus
2.3.1.32 1.35
-
 acetyl phosphate cosubstrate L-ornithine Bos taurus
2.3.1.32 4.24
-
 L-lysine
-
 Bos taurus
2.3.1.32 12.4
-
 acetyl phosphate cosubstrate L-lysine Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.32 Ca2+ slight stimulation Bos taurus
2.3.1.32 Mg2+ slight stimulation Bos taurus
2.3.1.32 additional information no activation by Zn2+, Mn2+, Co2+, Ni2+, Fe3+ or acetate Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.32 acetyl phosphate + L-lysine Bos taurus
-
 phosphate + N6-acetyl-L-lysine
-
 ?
2.3.1.32 acetyl phosphate + L-ornithine Bos taurus best substrate phosphate + N5-acetyl-L-ornithine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.32 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.32
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.32 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.32 1.43
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.3.1.32 0°C, 5 h Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.32 acetyl phosphate + L-lysine
-
 Bos taurus phosphate + N6-acetyl-L-lysine
-
 ?
2.3.1.32 acetyl phosphate + L-ornithine best substrate Bos taurus phosphate + N5-acetyl-L-ornithine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.32 38
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.32 8.6
-
 various buffer systems Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.32 8.3 9.2 about half-maximal activity at pH 8.3, about 70% of maximal activity at pH 9.2 Bos taurus