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Literature summary extracted from

  • Miyajima, R.; Shiio, I.
    Regulation of aspartate family amino acid biosynthesis in Brevibacterium flavum. VII. Properities of homoserine O-transacetylase (1973), J. Biochem., 73, 1061-1068.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.31 additional information methionine auxotroph strains show no activity [Brevibacterium] flavum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.31 L-homocysteine not [Brevibacterium] flavum
2.3.1.31 L-methionine no inhibition [Brevibacterium] flavum
2.3.1.31 additional information effects of several amino acids with and without addition of L-methionine, overview [Brevibacterium] flavum
2.3.1.31 O-acetyl-L-homoserine
-
 [Brevibacterium] flavum
2.3.1.31 S-adenosyl-L-methionine weak [Brevibacterium] flavum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.31 0.05
-
 acetyl-CoA
-
 [Brevibacterium] flavum
2.3.1.31 2.8
-
 L-homoserine
-
 [Brevibacterium] flavum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.31 acetyl-CoA + L-homoserine [Brevibacterium] flavum first reaction in L-methionine biosynthesis CoA + O-acetyl-L-homoserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.31 [Brevibacterium] flavum
-
 mutant strain M-116 derived from wild-type strain 2247
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.31 partial [Brevibacterium] flavum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.31 acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine ping-pong reaction mechanism [Brevibacterium] flavum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.31 0.143
-
-
 [Brevibacterium] flavum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.31 acetyl-CoA + L-homoserine succinyl-CoA cannot replace acetyl-CoA [Brevibacterium] flavum CoA + O-acetyl-L-homoserine
-
 ?
2.3.1.31 acetyl-CoA + L-homoserine first reaction in L-methionine biosynthesis [Brevibacterium] flavum CoA + O-acetyl-L-homoserine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.31 30
-
 assay at [Brevibacterium] flavum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.31 8
-
-
 [Brevibacterium] flavum