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Literature summary extracted from

  • Mino, K.; Hiraoka, K.; Imamura, K.; Sakiyama, T.; Eisaki, N.; Matsuyama, A.; Nakanishi, K.
    Characteristics of serine acetyltransferase from Escherichia coli deleting different lengths of amino acid residues from the C-terminus (2000), Biosci. Biotechnol. Biochem., 64, 1874-1880.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 C-terminal deletion mutants, expression in Escherichia coli strain JM70 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.30 additional information construction of deletion mutants lacking 10 to 25 amino acid residues at the C-terminal end, deletions alter the sensitivity against L-cysteine inhibition and the interaction with O-acetylserine (thiol) lyase, overview, DELTAC30 mutant is inactive Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.30 L-cysteine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.30 145000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Escherichia coli
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.30 recombinant wild-type and mutants from E. coli Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.30 acetyl-CoA + L-serine = CoA + O-acetyl-L-serine binding motif for O-acetylserine (thiol) lyase is located within 10 amino acid residues at the C-terminal end Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Escherichia coli CoA + O-acetyl-L-serine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.30 SAT
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.30 25
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.30
-
-
 SAT DELTAC10 mutant, lacking 10 amino acid residues at the C-terminal end is inactivated after 12 h Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.30 7.5
-
 assay at Escherichia coli