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Literature summary extracted from

  • Wirtz, M.; Berkowitz, O.; Droux, M.; Hell, R.
    The cysteine synthase complex from plants: mitochondrial serine acetyltransferase from Arabidopsis thaliana carries a bifunctional domain for catalysis and protein-protein interaction (2001), Eur. J. Biochem., 268, 686-693.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 construction of an inactivated mutant by targeted disruption of CysE gene for usage in complementation assays Escherichia coli
2.3.1.30 expression in Escherichia coli SAT inactivated mutant Arabidopsis thaliana
2.3.1.30 expression of wild-type and mutants in Escherichia coli SAT inactivated mutant as S-tagged protein Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.30 E282A site-directed mutagenesis in C-terminal hexapeptide-repeat domain, enhanced activity, complementation of inactivated Escherichia coli mutant Arabidopsis thaliana
2.3.1.30 G354A site-directed mutagenesis in C-terminal hexapeptide-repeat domain, reduced activity, partial complementation of inactivated Escherichia coli mutant Arabidopsis thaliana
2.3.1.30 H309A site-directed mutagenesis in C-terminal hexapeptide-repeat domain, strongly reduced activity Arabidopsis thaliana
2.3.1.30 H327A site-directed mutagenesis in C-terminal hexapeptide-repeat domain, nearly no activity Arabidopsis thaliana
2.3.1.30 V353E site-directed mutagenesis in C-terminal hexapeptide-repeat domain, nearly no activity Arabidopsis thaliana
2.3.1.30 V353E strain BB1 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.30 V353E mutant enzyme does not bind O-acetylserine (thiol) lyase Arabidopsis thaliana
2.3.1.30 V353E mutant enzyme does not bind O-acetylserine (thiol) lyase Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.30 0.145
-
 acetyl-CoA recombinant isozyme A, wild-type Arabidopsis thaliana
2.3.1.30 0.176
-
 acetyl-CoA recombinant isozyme A, mutant G354A Arabidopsis thaliana
2.3.1.30 2.4
-
 L-serine recombinant isozyme A, wild-type Arabidopsis thaliana
2.3.1.30 25.55
-
 L-serine recombinant isozyme A, mutant G354A Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.30 additional information
-
 amino acid sequence alignment Arabidopsis thaliana
2.3.1.30 additional information
-
 amino acid sequence alignment Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Escherichia coli
-
 CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine Arabidopsis thaliana
-
 CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine Salmonella enterica subsp. enterica serovar Typhimurium
-
 CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine Arabidopsis thaliana rate-limiting step of cysteine biosynthesis CoA + O-acetyl-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Arabidopsis thaliana Q39218 gene SAT1-6, mitochondrial isozyme
-
2.3.1.30 Arabidopsis thaliana Q39218 gene SAT-1, isoform A
-
2.3.1.30 Arabidopsis thaliana Q42538 gene SAT52, isoform C
-
2.3.1.30 Arabidopsis thaliana Q42588 gene SAT5, isoform B
-
2.3.1.30 Escherichia coli
-
 EC1801, gene CysE
-
2.3.1.30 Salmonella enterica subsp. enterica serovar Typhimurium P29847 gene CysE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.30 recombinant wild-type and mutants as S-tagged protein from E. coli Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.30 acetyl-CoA + L-serine = CoA + O-acetyl-L-serine a C-terminal hexapeptide-repeat domain is common for Arabidopsis thaliana isoforms and several other enzyme DNA sequences, it has a catalytic bifunctionality as serine acetyltransferase and in interaction with O-acetylserine (thiol) lyase and is involved in cysteine biosynthesis regulation, computational modeling Arabidopsis thaliana
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.30 0.00036
-
 purified recombinant mitochondrial isozyme A, mutant H327A Arabidopsis thaliana
2.3.1.30 0.00134
-
 purified recombinant mitochondrial isozyme A, mutant V353E Arabidopsis thaliana
2.3.1.30 0.00513
-
 purified recombinant mitochondrial isozyme A, mutant G354A Arabidopsis thaliana
2.3.1.30 0.032
-
 purified recombinant mitochondrial isozyme A, wild-type Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Escherichia coli CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine
-
 Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine rate-limiting step of cysteine biosynthesis Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.30 More serine acetyltransferase and O-acetylserine (thiol) lyase form the cysteine synthase complex Arabidopsis thaliana
2.3.1.30 SAT
-
 Escherichia coli
2.3.1.30 SAT
-
 Arabidopsis thaliana
2.3.1.30 SAT
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.30 25
-
 assay at Escherichia coli
2.3.1.30 25
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.30 7.5
-
 assay at Escherichia coli
2.3.1.30 7.5
-
 assay at Arabidopsis thaliana