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Literature summary extracted from

  • Toda, K.; Takano, H.; Miyagishima, S.y.; Kuroiwa, H.; Kuroiwa, T.
    Characterization of a chloroplast isoform of serine acetyltransferase from the thermo-acidiphilic red alga Cyanidioschyzon merolae (1998), Biochim. Biophys. Acta, 1403, 72-84.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 expression of His-tagged protein encoded on gene cmSAT in Escherichia coli, functional complementation of SAT-deficient Escherichia coli strain JM15, DNA and amino acid sequence determination Cyanidioschyzon merolae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.30 L-cysteine
-
 Cyanidioschyzon merolae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.30 chloroplast chloroplast isozyme, gene cmSAT Cyanidioschyzon merolae 9507
-
2.3.1.30 cytosol small amount of enzyme Cyanidioschyzon merolae 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.30 31000
-
 x * 31000, recombinant enzyme, SDS-PAGE Cyanidioschyzon merolae
2.3.1.30 43737
-
 x * 43737, native enzyme, amino acid sequence determination Cyanidioschyzon merolae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Cyanidioschyzon merolae
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Cyanidioschyzon merolae
-
 red alga
-
2.3.1.30 Cyanidioschyzon merolae
-
 chloroplast isoform, constitutive gene cmSAT
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.30 recombinant His-tagged protein from E. coli Cyanidioschyzon merolae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.30 46
-
 recombinant enzyme, crude extract Cyanidioschyzon merolae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Cyanidioschyzon merolae CoA + O-acetyl-L-serine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.30 ? x * 31000, recombinant enzyme, SDS-PAGE Cyanidioschyzon merolae
2.3.1.30 ? x * 43737, native enzyme, amino acid sequence determination Cyanidioschyzon merolae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.30 More serine acetyltransferase and O-acetylserine (thiol) lyase form the cysteine synthase complex Cyanidioschyzon merolae