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Literature summary extracted from

  • Droux, M.; Ruffet, M.L.; Douce, R.; Job, D.
    Interactions between serine acetyltransferase and O-acetylserine (thiol) lyase in higher plants. Structural and kinetic properties of the free and bound enzymes (1998), Eur. J. Biochem., 255, 235-245.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 overexpression in Escherichia coli Arabidopsis thaliana
2.5.1.47 expressed in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.30 additional information
-
 additional information cysteine synthase bienzyme complex Arabidopsis thaliana
2.3.1.30 0.91
-
 acetyl-CoA free recombinant enzyme Arabidopsis thaliana
2.3.1.30 2.84
-
 L-serine free recombinant enzyme Arabidopsis thaliana
2.5.1.47 0.37
-
 H2S pH 7.0, 25°C, recombinant free enzyme Arabidopsis thaliana
2.5.1.47 0.55
-
 H2S pH 7.5, 25°C, recombinant complex-bound enzyme Arabidopsis thaliana
2.5.1.47 0.64
-
 O-acetyl-L-Ser pH 7.5, 25°C, recombinant free enzyme Arabidopsis thaliana
2.5.1.47 4.28
-
 O-acetyl-L-Ser pH 7.5, 25°C, recombinant complex-bound enzyme Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.30 chloroplast
-
 Arabidopsis thaliana 9507
-
2.5.1.47 chloroplast bienzyme complex Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.30 34000
-
 2 * 34000, serine acetyltransferase + 2 * 36000, O-acetylserine (thiol) lyase, forming the cysteine synthase complex, SDS-PAGE Arabidopsis thaliana
2.3.1.30 36000
-
 2 * 34000, serine acetyltransferase + 2 * 36000, O-acetylserine (thiol) lyase, forming the cysteine synthase complex, SDS-PAGE Arabidopsis thaliana
2.3.1.30 300000
-
 cysteine synthase bienzyme complex, gel filtration Arabidopsis thaliana
2.3.1.30 2000000
-
 enzyme aggregate, gel filtration Arabidopsis thaliana
2.5.1.47 36000
-
 2 * 36000 Arabidopsis thaliana
2.5.1.47 300000
-
 bienzyme complex with serine acetyltransferase, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Arabidopsis thaliana regulatory role in cysteine biosynthesis CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 additional information Arabidopsis thaliana cysteine synthase is a bienzyme complex build of serine acetyltransferase and O-acetylserine (thiol) lyase, no channeling of substrate within the complex ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Arabidopsis thaliana
-
-
-
2.5.1.47 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.30 recombinant from E. coli Arabidopsis thaliana
2.5.1.47
-
 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.30 4.4
-
 recombinant protein, crude extract from E. coli Arabidopsis thaliana
2.3.1.30 70
-
 purified recombinant enzyme Arabidopsis thaliana
2.5.1.47 additional information
-
 activity modified by protein-protein interactions within cystein synthase complex Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine regulatory role in cysteine biosynthesis Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 additional information cysteine synthase is a bienzyme complex build of serine acetyltransferase and O-acetylserine (thiol) lyase, no channeling of substrate within the complex Arabidopsis thaliana ?
-
 ?
2.5.1.47 O-acetyl-L-serine
-
 Arabidopsis thaliana L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.30 tetramer 2 * 34000, serine acetyltransferase + 2 * 36000, O-acetylserine (thiol) lyase, forming the cysteine synthase complex, SDS-PAGE Arabidopsis thaliana
2.5.1.47 dimer 2 * 36000 Arabidopsis thaliana
2.5.1.47 More two dimeric enzyme molecules form complex with tetrameric serine acetyltransferase, complex does not dissociate during catalysis Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.1.30 More serine acetyltransferase and O-acetylserine (thiol) lyase form the cysteine synthase complex, consisting of 2 of each enzymes as a tetramer Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.30 pyridoxal 5'-phosphate 4 mol per mol of cysteine synthase complex Arabidopsis thaliana
2.5.1.47 pyridoxal 5'-phosphate 4 per cysteine synthase complex Arabidopsis thaliana