Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ruffet, M.L.; Droux, M.; Douce, R.
    Purification and kinetic properties of serine acetyltransferase free of O-acetylserine(thiol)lyase from spinach chloroplasts (1994), Plant Physiol., 104, 597-604.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.30 0.35
-
 acetyl-CoA
-
 Spinacia oleracea
2.3.1.30 2.29
-
 L-serine
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.30 chloroplast stroma Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.30 33000
-
 x * 33000, SDS-PAGE Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Spinacia oleracea first enzyme in L-cysteine synthesis pathway CoA + O-acetyl-L-serine
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.30
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.30 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.30 additional information
-
 coupled assay system Spinacia oleracea
2.3.1.30 205
-
 purified enzyme, pH 7.5 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.3.1.30 20°C, 0.4 M NaCl, 2 weeks, stable Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Spinacia oleracea CoA + O-acetyl-L-serine
-
 ir
2.3.1.30 acetyl-CoA + L-serine first enzyme in L-cysteine synthesis pathway Spinacia oleracea CoA + O-acetyl-L-serine
-
 ir

Subunits

EC Number Subunits Comment Organism
2.3.1.30 ? x * 33000, SDS-PAGE Spinacia oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.30 25
-
 assay at Spinacia oleracea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.30 15 70
-
 Spinacia oleracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.30 80
-
 5 min, 50% loss of activity Spinacia oleracea
2.3.1.30 90
-
 5 min, complete loss of activity Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.30 8 8.5
-
 Spinacia oleracea