Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chang, T.Y.; Chang, C.C.Y.; Cheng, D.
    Acyl-coenzyme A:cholesterol acyltransferase (1997), Annu. Rev. Biochem., 66, 613-638.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.26 25-hydroxycholesterol
-
 Homo sapiens
2.3.1.26 25-hydroxycholesterol
-
 Rattus norvegicus
2.3.1.26 cholesterol
-
 Cricetulus griseus
2.3.1.26 cholesterol
-
 Homo sapiens
2.3.1.26 cholesterol
-
 Rattus norvegicus
2.3.1.26 cholesterol exogenous, added as phosphatidylcholine liposome or in acetone solution, stimulates Platyrrhini
2.3.1.26 additional information regulation by sterol Cricetulus griseus
2.3.1.26 additional information regulation by sterol Cavia porcellus
2.3.1.26 additional information regulation by sterol Mus musculus
2.3.1.26 additional information regulation by sterol Homo sapiens
2.3.1.26 additional information regulation by sterol Rattus norvegicus
2.3.1.26 additional information regulation by sterol Sus scrofa
2.3.1.26 additional information regulation by sterol Oryctolagus cuniculus
2.3.1.26 additional information regulation by sterol Platyrrhini
2.3.1.26 additional information regulation by sterol Columba sp.

Application

EC Number Application Comment Organism
2.3.1.26 medicine identified as a major target for inhibition, based on the hypothesis that enzyme inhibitors may have a direct antiatherosclerotic effect within the arterial wall and a direct effect in blocking cholesterol absorption in the small intestine, and in reducing very-low-density lipoprotein secretion in the hepatocytes Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.26 cDNA cloned Cricetulus griseus
2.3.1.26 cDNA cloned Oryctolagus cuniculus
2.3.1.26 expression in enzyme-deficient mutant chinese hamster ovary cells Homo sapiens
2.3.1.26 expression in insect SF9 cells Homo sapiens
2.3.1.26 expression in Xenopus oocytes Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.26 Acetic anhydride 2 distinct tissue types with difference in reactivity, the inhibitor appears to modify a histidine residue Oryctolagus cuniculus
2.3.1.26 CI-976
-
 Homo sapiens
2.3.1.26 CI-976
-
 Oryctolagus cuniculus
2.3.1.26 diethyl dicarbonate 2 distinct tissue types with difference in reactivity, DEP-sensitive subtype typified by aortic ACTAT, DEP-resistant subtype typified by liver ACAT, irreversible inhibition, the inhibitor appear to modify a histidine residue Oryctolagus cuniculus
2.3.1.26 additional information regulation by non-sterol agents Cavia porcellus
2.3.1.26 additional information regulation by non-sterol agents Columba sp.
2.3.1.26 additional information regulation by non-sterol agents Cricetulus griseus
2.3.1.26 additional information regulation by non-sterol agents Homo sapiens
2.3.1.26 additional information regulation by non-sterol agents Mus musculus
2.3.1.26 additional information regulation by non-sterol agents; the inhibitor CI-976 given to cholesterol-fed rabbits prevents the accumulation of monocyte-macrophages within a pre-established iliac-femoral lesion and significantly reduces foam cell area Oryctolagus cuniculus
2.3.1.26 additional information regulation by non-sterol agents Platyrrhini
2.3.1.26 additional information regulation by non-sterol agents Rattus norvegicus
2.3.1.26 additional information regulation by non-sterol agents Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.26 endoplasmic reticulum
-
 Cricetulus griseus 5783
-
2.3.1.26 endoplasmic reticulum
-
 Homo sapiens 5783
-
2.3.1.26 endoplasmic reticulum
-
 Platyrrhini 5783
-
2.3.1.26 endoplasmic reticulum rough, membranes Rattus norvegicus 5783
-
2.3.1.26 membrane
-
 Mus musculus 16020
-
2.3.1.26 membrane
-
 Rattus norvegicus 16020
-
2.3.1.26 microsome
-
 Mus musculus
-
-
2.3.1.26 microsome
-
 Homo sapiens
-
-
2.3.1.26 microsome
-
 Rattus norvegicus
-
-
2.3.1.26 microsome
-
 Sus scrofa
-
-
2.3.1.26 microsome
-
 Oryctolagus cuniculus
-
-
2.3.1.26 additional information
-
 Platyrrhini
-
-
2.3.1.26 additional information cholesterol flux into and out of the endoplasmic reticulum Cricetulus griseus
-
-
2.3.1.26 additional information cholesterol flux into and out of the endoplasmic reticulum Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.26 64810
-
-
 Homo sapiens
2.3.1.26 213000
-
 approximately, liver membranes, radiation inactivation analysis Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Cricetulus griseus
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Cavia porcellus
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Mus musculus
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Homo sapiens
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Rattus norvegicus
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Sus scrofa
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Oryctolagus cuniculus
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Platyrrhini
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Columba sp.
-
 CoA + cholesteryl long-chain fatty acyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Cavia porcellus
-
-
-
2.3.1.26 Columba sp.
-
 pigeon
-
2.3.1.26 Cricetulus griseus
-
-
-
2.3.1.26 Homo sapiens
-
-
-
2.3.1.26 Mus musculus
-
-
-
2.3.1.26 Oryctolagus cuniculus
-
-
-
2.3.1.26 Platyrrhini
-
-
-
2.3.1.26 Rattus norvegicus
-
-
-
2.3.1.26 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.26
-
 Sus scrofa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.26 solubilization of enzyme activity by deoxycholate, reconstitution of the solubilized activity into liposome of known cholesterol and phospholipid content by employing a cholate dialysis procedure Cricetulus griseus
2.3.1.26 solubilization of enzyme activity by deoxycholate, reconstitution of the solubilized activity into liposome of known cholesterol and phospholipid content by employing a cholate dialysis procedure Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.26 adrenal gland
-
 Homo sapiens
-
2.3.1.26 adrenal gland
-
 Platyrrhini
-
2.3.1.26 adrenal gland relative high level of mRNA Mus musculus
-
2.3.1.26 adrenal gland relative high level of mRNA Rattus norvegicus
-
2.3.1.26 adrenal gland relative high level of mRNA Oryctolagus cuniculus
-
2.3.1.26 aorta
-
 Mus musculus
-
2.3.1.26 aorta
-
 Rattus norvegicus
-
2.3.1.26 aorta atheroschlerotic tissue Oryctolagus cuniculus
-
2.3.1.26 brain
-
 Mus musculus
-
2.3.1.26 brain
-
 Homo sapiens
-
2.3.1.26 brain
-
 Oryctolagus cuniculus
-
2.3.1.26 CHO cell
-
 Cricetulus griseus
-
2.3.1.26 heart
-
 Mus musculus
-
2.3.1.26 heart
-
 Homo sapiens
-
2.3.1.26 heart
-
 Oryctolagus cuniculus
-
2.3.1.26 heart
-
 Platyrrhini
-
2.3.1.26 kidney
-
 Homo sapiens
-
2.3.1.26 kidney
-
 Oryctolagus cuniculus
-
2.3.1.26 kidney
-
 Platyrrhini
-
2.3.1.26 liver
-
 Cavia porcellus
-
2.3.1.26 liver
-
 Mus musculus
-
2.3.1.26 liver
-
 Homo sapiens
-
2.3.1.26 liver
-
 Sus scrofa
-
2.3.1.26 liver
-
 Platyrrhini
-
2.3.1.26 liver
-
 Columba sp.
-
2.3.1.26 liver the enzyme activity is very low in fetal livers but is largely induced shortly after birth Rattus norvegicus
-
2.3.1.26 liver nonparenchymal cells contain 30fold more enzyme message than parenchymal cells Oryctolagus cuniculus
-
2.3.1.26 macrophage
-
 Homo sapiens
-
2.3.1.26 macrophage relative high level of mRNA Mus musculus
-
2.3.1.26 macrophage relative high level of mRNA Oryctolagus cuniculus
-
2.3.1.26 peritoneal macrophage
-
 Cricetulus griseus
-
2.3.1.26 peritoneal macrophage
-
 Cavia porcellus
-
2.3.1.26 peritoneal macrophage messages relative abundant Mus musculus
-
2.3.1.26 peritoneal macrophage messages relative abundant Rattus norvegicus
-
2.3.1.26 peritoneal macrophage messages relative abundant Oryctolagus cuniculus
-
2.3.1.26 preputial gland highly expressed Mus musculus
-
2.3.1.26 skeletal muscle
-
 Mus musculus
-
2.3.1.26 skeletal muscle
-
 Homo sapiens
-
2.3.1.26 testis relative high level of mRNA Mus musculus
-
2.3.1.26 testis relative high level of mRNA Rattus norvegicus
-
2.3.1.26 testis relative high level of mRNA Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.26 additional information
-
-
 Cricetulus griseus
2.3.1.26 additional information
-
-
 Cavia porcellus
2.3.1.26 additional information
-
-
 Mus musculus
2.3.1.26 additional information
-
-
 Homo sapiens
2.3.1.26 additional information
-
-
 Rattus norvegicus
2.3.1.26 additional information
-
-
 Sus scrofa
2.3.1.26 additional information
-
-
 Platyrrhini
2.3.1.26 additional information
-
-
 Columba sp.
2.3.1.26 additional information
-
 enhancement of activity following 8-11 weeks of cholesterol feeding Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
 Cricetulus griseus CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
 Cavia porcellus CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
 Mus musculus CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
 Homo sapiens CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
 Rattus norvegicus CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
 Sus scrofa CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
 Oryctolagus cuniculus CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
 Platyrrhini CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol
-
 Columba sp. CoA + cholesteryl long-chain fatty acyl ester
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.26 ? x * 45000-50000, SDS-PAGE Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.26 CoA
-
 Homo sapiens
2.3.1.26 CoA required by the in-situ acyl-CoA production Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.26 0.04
-
 diethyl dicarbonate aortic enzyme Oryctolagus cuniculus
2.3.1.26 0.5
-
 Acetic anhydride less than, aortic enzyme Oryctolagus cuniculus
2.3.1.26 1.5
-
 diethyl dicarbonate liver enzyme Oryctolagus cuniculus
2.3.1.26 5
-
 Acetic anhydride greater than, liver enzyme Oryctolagus cuniculus