Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Erickson, S.K.; Shrewsbury, M.A.; Brooks, C.; Meyer, D.J.
    Rat liver acyl-coenzyme A:cholesterol acyltransferase: its regulation in vivo and some of its properties in vitro (1980), J. Lipid Res., 21, 930-941.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.26 25-hydroxycholesterol activates Rattus norvegicus
2.3.1.26 cholate increase of activity in cholate-fed animals Rattus norvegicus
2.3.1.26 cholesterol increase of activity in cholesterol-fed animals Rattus norvegicus
2.3.1.26 Mevalonolactone intragastric administration, increasing sterol biosynthesis and increasing enzyme activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.26 acetone 30-50% inhibition at a final concentration of 1% v/v, inhibition prevented by the addition of cholesterol to the solvent Rattus norvegicus
2.3.1.26 butanol 30-50% inhibition at a final concentration of 1% v/v, inhibition prevented by the addition of cholesterol to the solvent Rattus norvegicus
2.3.1.26 Detergents above their critical micelle concentration, e.g. cholate, deoxycholate, cetyltriethylammonium bromide, Triton X-100, Tween 20, Tween 80, Nonidet P40 Rattus norvegicus
2.3.1.26 Digitonin
-
 Rattus norvegicus
2.3.1.26 ethanol 30-50% inhibition at a final concentration of 1% v/v, inhibition prevented by the addition of cholesterol to the solvent Rattus norvegicus
2.3.1.26 Filipin
-
 Rattus norvegicus
2.3.1.26 oleoyl-CoA inhibits at high concentrations, the inhibition is prevented by serum albumin Rattus norvegicus
2.3.1.26 organic solvents
-
 Rattus norvegicus
2.3.1.26 progesterone
-
 Rattus norvegicus
2.3.1.26 SC-31769 20-oxa analog of 7-ketosterol Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.26 cytosol little activity Rattus norvegicus 5829
-
2.3.1.26 endoplasmic reticulum rough, membranes Rattus norvegicus 5783
-
2.3.1.26 microsome intrinsic membrane enzyme, highest activity Rattus norvegicus
-
-
2.3.1.26 mitochondrion little activity Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Rattus norvegicus the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol Rattus norvegicus male Sprague-Dawley the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis CoA + cholesteryl long-chain fatty acyl ester
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.3.1.26 additional information the use of organic solvents or strong detergents for the suspension of cholesterol must be avoided because of their affect on the enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Rattus norvegicus
-
 male Sprague-Dawley strain
-
2.3.1.26 Rattus norvegicus male Sprague-Dawley
-
 male Sprague-Dawley strain
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.26 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.26 additional information
-
 overview: effect of fasting, feeding with cholesterol or cholate, atherogenic diet administration of Triton WR 1339 and administration of mevanolactone, evidence for a circadian rhythm Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.26 frozen, stable for up to 2 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 acyl-CoA + cholesterol
-
 Rattus norvegicus CoA + cholesterol ester
-
 ?
2.3.1.26 acyl-CoA + cholesterol
-
 Rattus norvegicus male Sprague-Dawley CoA + cholesterol ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis Rattus norvegicus CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 long-chain fatty acyl-CoA + cholesterol the enzyme plays an important role in maintenance of hepatic cholesterol homeostasis Rattus norvegicus male Sprague-Dawley CoA + cholesteryl long-chain fatty acyl ester
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol
-
 Rattus norvegicus CoA + cholesteryl oleate
-
 ?
2.3.1.26 oleoyl-CoA + cholesterol
-
 Rattus norvegicus male Sprague-Dawley CoA + cholesteryl oleate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.26 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.26 80
-
 10 min, complete loss of activity Rattus norvegicus