BRENDA - Enzyme Database

Lysophosphatidylcholine acyltransferase activity in Saccharomyces cerevisiae: regulation by a high-affinity Zn2+ binding site

Richard, M.G.; McMaster, C.R.; Lipids 33, 1229-1234 (1998)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.23
cardiolipin
236% activation
Saccharomyces cerevisiae
2.3.1.23
phosphatidic acid
182% activation
Saccharomyces cerevisiae
2.3.1.23
phosphatidylinositol
212% activation
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.23
o-phenanthroline
2 mM, 82% inhibition, 1 mM, 73% inhibition
Saccharomyces cerevisiae
2.3.1.23
Zn2+
0.1 mM, 67% inhibition, 1 mM, 93% inhibition
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.23
0.069
-
1-acyl-sn-glycero-3-phosphocholine
-
Saccharomyces cerevisiae
2.3.1.23
0.152
-
oleoyl-CoA
-
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.23
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.23
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
486651
Saccharomyces cerevisiae
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
486651
Saccharomyces cerevisiae
r
2.3.1.23
acyl-CoA + 1-O-alkyl-sn-glycero-3-phosphocholine
-
486651
Saccharomyces cerevisiae
CoA + 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine
-
486651
Saccharomyces cerevisiae
?
2.3.1.23
acyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine
preferred acyl acceptors in descending order: 1-oleoyl-sn-glycero-3-phosphocholine, 1-myristoyl-sn-glycero-3-phosphocholine, 1-lauroyl-sn-glycero-3-phosphocholine, 1-stearoyl-sn-glycero-3-phosphocholine, 1-palmitoyl-sn-glycero-3-phosphocholine, 1-decanoyl-sn-glycero-3-phosphocholine and 1-arachidonoyl-sn-glycero-3-phosphocholine
486651
Saccharomyces cerevisiae
CoA + 1-oleoyl-2-acyl-sn-glycero-3-phosphocholine
-
486651
Saccharomyces cerevisiae
?
2.3.1.23
oleoyl-CoA + lysophosphatidyl choline
-
486651
Saccharomyces cerevisiae
?
-
-
-
-
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.23
cardiolipin
236% activation
Saccharomyces cerevisiae
2.3.1.23
phosphatidic acid
182% activation
Saccharomyces cerevisiae
2.3.1.23
phosphatidylinositol
212% activation
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.23
o-phenanthroline
2 mM, 82% inhibition, 1 mM, 73% inhibition
Saccharomyces cerevisiae
2.3.1.23
Zn2+
0.1 mM, 67% inhibition, 1 mM, 93% inhibition
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.23
0.069
-
1-acyl-sn-glycero-3-phosphocholine
-
Saccharomyces cerevisiae
2.3.1.23
0.152
-
oleoyl-CoA
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.23
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
486651
Saccharomyces cerevisiae
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
486651
Saccharomyces cerevisiae
r
2.3.1.23
acyl-CoA + 1-O-alkyl-sn-glycero-3-phosphocholine
-
486651
Saccharomyces cerevisiae
CoA + 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine
-
486651
Saccharomyces cerevisiae
?
2.3.1.23
acyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine
preferred acyl acceptors in descending order: 1-oleoyl-sn-glycero-3-phosphocholine, 1-myristoyl-sn-glycero-3-phosphocholine, 1-lauroyl-sn-glycero-3-phosphocholine, 1-stearoyl-sn-glycero-3-phosphocholine, 1-palmitoyl-sn-glycero-3-phosphocholine, 1-decanoyl-sn-glycero-3-phosphocholine and 1-arachidonoyl-sn-glycero-3-phosphocholine
486651
Saccharomyces cerevisiae
CoA + 1-oleoyl-2-acyl-sn-glycero-3-phosphocholine
-
486651
Saccharomyces cerevisiae
?
2.3.1.23
oleoyl-CoA + lysophosphatidyl choline
-
486651
Saccharomyces cerevisiae
?
-
-
-
-