BRENDA - Enzyme Database

Purification and properties of acyl-CoA:1-acyl-sn-glycero-3-phosphocholine-O-acyltransferase from bovine brain microsomes

Deka, N.; Sun, G.Y.; MacQuarrie, R.; Arch. Biochem. Biophys. 246, 554-563 (1986) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.23
Arachidonoyl-CoA
high concentration
Bos taurus
2.3.1.23
Trypsin
inclusion in liposomes protects
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.23
additional information
-
additional information
kinetic data of soluble and liposome-bound enzyme
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.23
microsome
-
Bos taurus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.23
43000
-
x * 43000, brain enzyme, SDS-PAGE
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.23
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
Bos taurus
participates in deacylation/reacylation cycle of cell membranes
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
Bos taurus
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.23
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.23
solubilized with lysolecithin, reactive Blue-2-agarose, DEAE cellulose, Matrex Green A
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.23
brain
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.23
0.079
-
acyl donor oleoyl-CoA
Bos taurus
2.3.1.23
0.134
-
acyl donor linoleoyl-CoA
Bos taurus
2.3.1.23
0.61
-
acyl donor arachidonoyl-CoA
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.23
-20°C, liposome-bound enzyme, at least 10 h
Bos taurus
2.3.1.23
-20°C, soluble enzyme, less than 10 h
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.23
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
participates in deacylation/reacylation cycle of cell membranes
486624
Bos taurus
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
?
2.3.1.23
acyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
no activity with 1-myristoyl-sn-glycero-3-phosphocholine
486624
Bos taurus
CoA + 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
acyl-CoA + 1-stearoyl-sn-glycero-3-phosphocholine
no activity with 1-myristoyl-sn-glycero-3-phosphocholine
486624
Bos taurus
CoA + 1-stearoyl-2-acyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
preferred acyl donor
486624
Bos taurus
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
cis-11-eicosaenoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
13% of activity with arachidonoyl-CoA
486624
Bos taurus
CoA + 1-acyl-2-cis-11-eicosaenoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
decanoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
58% of activity with arachidonoyl-CoA
486624
Bos taurus
CoA + 1-acyl-2-decanoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
eicosatrienoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
20% of activity with arachidonoyl-CoA
486624
Bos taurus
CoA + 1-acyl-2-eicosatrienoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
linolenoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
8% of activity with arachidonoyl-CoA
486624
Bos taurus
CoA + 1-acyl-2-linolenoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
myristoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
41% of activity with arachidonoyl-CoA
486624
Bos taurus
CoA + 1-acyl-2-myristoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.23
?
x * 43000, brain enzyme, SDS-PAGE
Bos taurus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.23
37
-
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.23
Arachidonoyl-CoA
high concentration
Bos taurus
2.3.1.23
Trypsin
inclusion in liposomes protects
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.23
additional information
-
additional information
kinetic data of soluble and liposome-bound enzyme
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.23
microsome
-
Bos taurus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.23
43000
-
x * 43000, brain enzyme, SDS-PAGE
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.23
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
Bos taurus
participates in deacylation/reacylation cycle of cell membranes
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
Bos taurus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.23
solubilized with lysolecithin, reactive Blue-2-agarose, DEAE cellulose, Matrex Green A
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.23
brain
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.23
0.079
-
acyl donor oleoyl-CoA
Bos taurus
2.3.1.23
0.134
-
acyl donor linoleoyl-CoA
Bos taurus
2.3.1.23
0.61
-
acyl donor arachidonoyl-CoA
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.23
-20°C, liposome-bound enzyme, at least 10 h
Bos taurus
2.3.1.23
-20°C, soluble enzyme, less than 10 h
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.23
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
participates in deacylation/reacylation cycle of cell membranes
486624
Bos taurus
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
?
2.3.1.23
acyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
no activity with 1-myristoyl-sn-glycero-3-phosphocholine
486624
Bos taurus
CoA + 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
acyl-CoA + 1-stearoyl-sn-glycero-3-phosphocholine
no activity with 1-myristoyl-sn-glycero-3-phosphocholine
486624
Bos taurus
CoA + 1-stearoyl-2-acyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
preferred acyl donor
486624
Bos taurus
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
cis-11-eicosaenoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
13% of activity with arachidonoyl-CoA
486624
Bos taurus
CoA + 1-acyl-2-cis-11-eicosaenoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
decanoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
58% of activity with arachidonoyl-CoA
486624
Bos taurus
CoA + 1-acyl-2-decanoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
eicosatrienoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
20% of activity with arachidonoyl-CoA
486624
Bos taurus
CoA + 1-acyl-2-eicosatrienoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
linolenoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
8% of activity with arachidonoyl-CoA
486624
Bos taurus
CoA + 1-acyl-2-linolenoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
myristoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
41% of activity with arachidonoyl-CoA
486624
Bos taurus
CoA + 1-acyl-2-myristoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
2.3.1.23
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
486624
Bos taurus
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.23
?
x * 43000, brain enzyme, SDS-PAGE
Bos taurus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.23
37
-
-
Bos taurus