BRENDA - Enzyme Database

Identification, purification, and characterization of monoacylglycerol acyltransferase from developing peanut cotyledons

Tumaney, A.W.; Shekar, S.; Rajasekharan, R.; J. Biol. Chem. 276, 10847-10852 (2001)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.22
oleic acid
stimulates below 0.015 mM
Arachis hypogaea
2.3.1.22
palmitic acid
0.02-0.05 mM, stimulates
Arachis hypogaea
2.3.1.22
phosphatidic acid
stimulates
Arachis hypogaea
2.3.1.22
phosphatidylcholine
stimulates
Arachis hypogaea
2.3.1.22
sphingomyelin
0.0025-0.0075 mM, activates
Arachis hypogaea
2.3.1.22
sphingosine
0.0025-0.0075 mM, activates
Arachis hypogaea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.22
beta-octylglucoside
40 mM, 50% loss of activity
Arachis hypogaea
2.3.1.22
CHAPS
20 mM, complete loss of activity
Arachis hypogaea
2.3.1.22
NaF
-
Arachis hypogaea
2.3.1.22
Triton X-100
42% loss of activity
Arachis hypogaea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.22
0.00565
-
1-oleoylglycerol
-
Arachis hypogaea
2.3.1.22
0.00935
-
oleoyl-CoA
-
Arachis hypogaea
2.3.1.22
0.01639
-
1-palmitoylglycerol
-
Arachis hypogaea
2.3.1.22
0.01754
-
palmitoyl-CoA
-
Arachis hypogaea
2.3.1.22
0.02566
-
stearoyl-CoA
-
Arachis hypogaea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.22
43000
-
gel filtration
Arachis hypogaea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.22
acyl-CoA + sn-2-monoacylglycerol
Arachis hypogaea
diacylglycerol biosynthetic activity
CoA + diacylglycerol
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.22
Arachis hypogaea
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.22
-
Arachis hypogaea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.22
cotyledon
-
Arachis hypogaea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.22
0.0159
-
-
Arachis hypogaea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.22
acyl-CoA + sn-2-monoacylglycerol
diacylglycerol biosynthetic activity
486606
Arachis hypogaea
CoA + diacylglycerol
-
-
-
-
2.3.1.22
oleoyl-CoA + 1-palmitoyl-sn-glycerol
-
486606
Arachis hypogaea
CoA + 1-palmitoyl-3-oleoyl-sn-glycerol
-
-
-
?
2.3.1.22
palmitoyl-CoA + 1-palmitoyl-sn-glycerol
-
486606
Arachis hypogaea
CoA + ?
-
-
-
?
2.3.1.22
palmitoyl-CoA + sn-1-monooleoylglycerol
-
486606
Arachis hypogaea
CoA + ?
-
-
-
?
2.3.1.22
stearoyl-CoA + 1-palmitoyl-sn-glycerol
-
486606
Arachis hypogaea
CoA + ?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.22
monomer
1 * 43000, SDS-PAGE
Arachis hypogaea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.22
7
-
-
Arachis hypogaea
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.22
oleic acid
stimulates below 0.015 mM
Arachis hypogaea
2.3.1.22
palmitic acid
0.02-0.05 mM, stimulates
Arachis hypogaea
2.3.1.22
phosphatidic acid
stimulates
Arachis hypogaea
2.3.1.22
phosphatidylcholine
stimulates
Arachis hypogaea
2.3.1.22
sphingomyelin
0.0025-0.0075 mM, activates
Arachis hypogaea
2.3.1.22
sphingosine
0.0025-0.0075 mM, activates
Arachis hypogaea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.22
beta-octylglucoside
40 mM, 50% loss of activity
Arachis hypogaea
2.3.1.22
CHAPS
20 mM, complete loss of activity
Arachis hypogaea
2.3.1.22
NaF
-
Arachis hypogaea
2.3.1.22
Triton X-100
42% loss of activity
Arachis hypogaea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.22
0.00565
-
1-oleoylglycerol
-
Arachis hypogaea
2.3.1.22
0.00935
-
oleoyl-CoA
-
Arachis hypogaea
2.3.1.22
0.01639
-
1-palmitoylglycerol
-
Arachis hypogaea
2.3.1.22
0.01754
-
palmitoyl-CoA
-
Arachis hypogaea
2.3.1.22
0.02566
-
stearoyl-CoA
-
Arachis hypogaea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.22
43000
-
gel filtration
Arachis hypogaea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.22
acyl-CoA + sn-2-monoacylglycerol
Arachis hypogaea
diacylglycerol biosynthetic activity
CoA + diacylglycerol
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.22
-
Arachis hypogaea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.22
cotyledon
-
Arachis hypogaea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.22
0.0159
-
-
Arachis hypogaea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.22
acyl-CoA + sn-2-monoacylglycerol
diacylglycerol biosynthetic activity
486606
Arachis hypogaea
CoA + diacylglycerol
-
-
-
-
2.3.1.22
oleoyl-CoA + 1-palmitoyl-sn-glycerol
-
486606
Arachis hypogaea
CoA + 1-palmitoyl-3-oleoyl-sn-glycerol
-
-
-
?
2.3.1.22
palmitoyl-CoA + 1-palmitoyl-sn-glycerol
-
486606
Arachis hypogaea
CoA + ?
-
-
-
?
2.3.1.22
palmitoyl-CoA + sn-1-monooleoylglycerol
-
486606
Arachis hypogaea
CoA + ?
-
-
-
?
2.3.1.22
stearoyl-CoA + 1-palmitoyl-sn-glycerol
-
486606
Arachis hypogaea
CoA + ?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.22
monomer
1 * 43000, SDS-PAGE
Arachis hypogaea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.22
7
-
-
Arachis hypogaea