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Literature summary extracted from

  • Masterson, C.; Wood, C.
    Carnitine palmitoyltransferases in pea leaf chloroplasts: partial purification, location, and properties (2000), Can. J. Bot., 78, 328-335.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.21 malonyl-CoA inhibits CPT I not CPT II; regulatory role in vivo Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.21 chloroplast inner envelope membrane with isoform CPT I on the outer, cytosolic side and isoform CPT II on the inner, stromal side of the membrane Pisum sativum 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.21 20000
-
 x * 20000, CPT I and II, SDS-PAGE Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine Pisum sativum enzyme has a key function in regulation of fatty acid beta-oxidation CoA + L-acylcarnitine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 Pisum sativum
-
 2 isoforms CPTo (I) and CPTi (II)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.21 partial, CPT I and II Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.21 leaf CPT I and CPT II Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.21 0.0073
-
 solubilized CPT Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine substrate specificity Pisum sativum CoA + L-acylcarnitine
-
 r
2.3.1.21 acyl-CoA + L-carnitine preference for desaturated long-chain acyl CoAs Pisum sativum CoA + L-acylcarnitine
-
 r
2.3.1.21 acyl-CoA + L-carnitine C4 to C18 acyl-CoAs Pisum sativum CoA + L-acylcarnitine
-
 r
2.3.1.21 acyl-CoA + L-carnitine enzyme has a key function in regulation of fatty acid beta-oxidation Pisum sativum CoA + L-acylcarnitine
-
 r
2.3.1.21 linoleoyl-CoA + L-carnitine preference for Pisum sativum CoA + L-linoleoylcarnitine
-
 r
2.3.1.21 palmitoyl-CoA + DL-carnitine
-
 Pisum sativum CoA + DL-palmitoylcarnitine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.21 ? x * 20000, CPT I and II, SDS-PAGE Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.21 27
-
 assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.21 7.5
-
 assay at Pisum sativum