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Literature summary extracted from

  • De Vries, Y.; Arvidson, D.N.; Waterham, H.R.; Cregg, J.M.; Woldegiorgis, G.
    Functional characterization of mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris (1997), Biochemistry, 36, 5285-5292.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.21 clonig of CPT I and II from liver, expression in Pichia pastoris Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.21 malonyl-CoA CPT I; CPT I catalytic activity and malonyl-CoA sensitivity is located on 1 single polypeptide; CPT II not inhibited; recombinant CPT I is highly sensitive, recombinant CPT II not Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.21 0.105
-
 L-carnitine recombinant CPT II Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.21 mitochondrial outer membrane CPT I is located in the outer membrane Rattus norvegicus 5741
-
2.3.1.21 mitochondrion CPT II Rattus norvegicus 5739
-
2.3.1.21 mitochondrion CPT I Rattus norvegicus 5739
-
2.3.1.21 additional information forward reaction by CPT I at outer mitochondrial membrane, transport of acylcarnitine through mitochondrial membrane, reverse reaction at inner face of inner membrane by CPT II, overview Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.21 70000
-
 x * 70000, recombinant CPT II, SDS-PAGE Rattus norvegicus
2.3.1.21 88000
-
 x * 88000, recombinant CPT I, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine Rattus norvegicus CPT I is the rate-limiting enzyme in beta-oxidation of long-chain fatty acids CoA + L-acylcarnitine
-
 ?
2.3.1.21 palmitoyl-CoA + L-carnitine Rattus norvegicus forward reaction by CPT I and reverse reaction by CPT II L-palmitoylcarnitine + CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 no activity in Pichia pastoris
-
-
-
2.3.1.21 Rattus norvegicus
-
 enzyme form CPT I
-
2.3.1.21 Rattus norvegicus
-
 enzyme form CPT II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.21 partial, recombinant CPT I and II from Pichia pastoris Rattus norvegicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.21 reconstitution of detergent-inactivated recombinant M-CPT I, purified from Pichia pastoris, by removal of detergents in presence of phospholipids Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.21 liver CPT II Rattus norvegicus
-
2.3.1.21 liver CPT I Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.21 0.0032
-
 mitochondrial fraction of recombinant Pichia pastoris expressing CPT I Rattus norvegicus
2.3.1.21 0.0069
-
 mitochondrial fraction of recombinant Pichia pastoris expressing CPT II Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 acyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-acylcarnitine
-
 ?
2.3.1.21 acyl-CoA + L-carnitine CPT I is the rate-limiting enzyme in beta-oxidation of long-chain fatty acids Rattus norvegicus CoA + L-acylcarnitine
-
 ?
2.3.1.21 palmitoyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-palmitoylcarnitine
-
 r
2.3.1.21 palmitoyl-CoA + L-carnitine forward reaction by CPT I and reverse reaction by CPT II Rattus norvegicus L-palmitoylcarnitine + CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.21 ? x * 88000, recombinant CPT I, SDS-PAGE Rattus norvegicus
2.3.1.21 ? x * 70000, recombinant CPT II, SDS-PAGE Rattus norvegicus